TCDB is operated by the Saier Lab Bioinformatics Group
« See all members of the family


3.A.1.1.53
Oligosaccharide transporter RafEFGK. RafE, the binding protein, has be extensively characterized.  It binds α-(1,6)-linked glucosides and galactosides of varying size, linkage, and monosaccharide composition with preference for the trisaccharides raffinose and panose. This preference is reflected in the α-(1,6)-galactoside uptake profile of the bacterium. Structures of RafE (BlG16BP) in complex with raffinose and panose revealed the basis for the ligand binding plasticity, which recognizes the non-reducing α-(1,6)-diglycosidic linkages in its ligands (Ejby et al. 2016). RafK has not be identified experimentally, but it may be NCIB protein acc# WP_022543180.1, ATP binding protein, annotated as UgpC, and this protein has been enterred into TCDB as RafK. Sugar binding substrates of RafE include: raffinose (highest affinity), panose, melibiose, stachyose, verbascose, isomaltose, isomaltotriose, isomaltotetraose, isomaltopentaose, isomaltohexaose, and isomaltoheptaose (Ejby et al. 2016).

Accession Number:D3R799
Protein Name:Raffinose-binding protein
Length:439
Molecular Weight:48657.00
Species:Bifidobacterium animalis subsp. lactis (strain BB-12) [552531]
Number of TMSs:1
Substrate

Cross database links:

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
Window Size: Angle:  
Window Size: Angle:  
FASTA formatted sequence
1:	MEMDKGVRMK FATRMRRSAM RRMLRGIGAA VCALAVGVGA AGCGNSAGSG QVTLDFFQFK 
61:	AEAADWFKQA AQEFEKENPD IRININNSAN AQTDLRTRFV KDRVPDVITF NGDYSFGTFA 
121:	ASGVFHDFTD DPLVSELNEG MVNIAKNLVQ TSDPAKKRLY GLPFAGNASG YIYNKDLFRK 
181:	VGLDPDNPPQ TWDEFIAMLK KFRDAGINPV QATLADAWTT QAPLASLAGT LVPESEYAAL 
241:	KSGDTTFKQI WTEPIEKEIE LFKYADSEKG VTYQQGTQNF AKGTAAIIPL GTYAIPQITM 
301:	VNKDIDLGFA QMPATNDASK QILTAGDDVI LTMGANSRHK EQSMRFIRFL MSKKQLENYA 
361:	DAQSAITPLK ETYFGNKALE PVRPFFESNR VADFCDHYIP SSINIGGYLQ SAIMSGNVNQ 
421:	FIDSMQNEWN KVQARDFRK