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3.A.19.1.1
The ATP hydrolysis-dependent TRC receptor TRC40 (Asna-1) (Stefanovic and Hegde, 2007). (TRC40 is homologous to the ArsA ATPase of E. coli (TC# 3.A.4.1.1) and the GET3 ATPase of yeast (TC# 8.A.26.1.1)) Loss yields embryonic lethality. Tryptophan-rich basic protein (WRB) is the tail-anchored (TA) protein insertion receptor. Also called congenital heart disease protein-5 (CHD5). Related to the yeast Get1 protein in 3.A.21.1.1. Calcium-modulating cyclophilin ligand (CAML) is a mammal-specific receptor for TRC40, an ATPase targeting newly synthesized TA proteins.  CAML mediates membrane insertion of TA proteins.  TRC40 (Asna1) has been shown to mediate membrane insertion of two proteins, RAMP4 and Sec61beta, without the participation of other cytosolic proteins by a mechanism that depends on the presence of ATP or ADP and a protease-sensitive receptor in the ER membrane (Favaloro et al. 2010).  TRC40 is required for release of Herpes simplex virus 1 (HSV1) virions (Ott et al. 2016). The functions and reciprocal interactions of the two subunits of the heteromeric TRC40 recpeptor, WBR and CAML (CAMLG), have revealed mutual dependencies for stability; CAML seems to normally be present in 5-fold excess over WBR (Colombo et al. 2016). CAMLG interacts with Classical Swine Fever Virus (CSFV) p7 and mediates calcium permeability in the ER (Gladue et al. 2018).

Accession Number:O00258
Protein Name:Tail-anchored protein insertion receptor WRB
Length:174
Molecular Weight:19780.00
Species:Homo sapiens (Human) [9606]
Number of TMSs:3
Location1 / Topology2 / Orientation3: Endoplasmic reticulum membrane1 / Multi-pass membrane protein2
Substrate tail-anchored proteins

Cross database links:

Genevestigator: O00258
eggNOG: NOG287621
HEGENOM: HOG000013223
Entrez Gene ID: 7485   
Pfam: PF04420   
Drugbank: Drugbank Link   
KEGG: hsa:7485   

Gene Ontology

GO:0005789 C:endoplasmic reticulum membrane
GO:0016021 C:integral to membrane
GO:0005730 C:nucleolus

References (5)

[1] “Identification and characterization of a new human cDNA from chromosome 21q22.3 encoding a basic nuclear protein.”  Egeo A.et.al.   9544840
[2] “Complete sequencing and characterization of 21,243 full-length human cDNAs.”  Ota T.et.al.   14702039
[3] “The DNA sequence of human chromosome 21.”  Hattori M.et.al.   10830953
[4] “The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).”  The MGC Project Teamet.al.   15489334
[5] “WRB is the receptor for TRC40/Asna1-mediated insertion of tail-anchored proteins into the ER membrane.”  Vilardi F.et.al.   21444755

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FASTA formatted sequence
1:	MSSAAADHWA WLLVLSFVFG CNVLRILLPS FSSFMSRVLQ KDAEQESQMR AEIQDMKQEL 
61:	STVNMMDEFA RYARLERKIN KMTDKLKTHV KARTAQLAKI KWVISVAFYV LQAALMISLI 
121:	WKYYSVPVAV VPSKWITPLD RLVAFPTRVA GGVGITCWIL VCNKVVAIVL HPFS