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2.A.6.2.9
Solvent efflux pump, TtgABC (extrudes toluene, styrene, m-xylene, ethylbenzene, acetate, α-pinene and propylbenzene) (Teran et al., 2007; Dunlop et al. 2011Dunlop et al. 2011).

Accession Number:O52248
Protein Name:TtgB
Length:1050
Molecular Weight:112808.00
Species:Pseudomonas putida [303]
Number of TMSs:12
Location1 / Topology2 / Orientation3: Cell inner membrane1 / Multi-pass membrane protein2
Substrate Toluene, Styrene, m-xylene, Acetate, Alpha-pinene, Propylbenzene

Cross database links:

Pfam: PF00873   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0005215 F:transporter activity
GO:0046677 P:response to antibiotic
GO:0006810 P:transport

References (3)

[1] “Efflux pumps involved in toluene tolerance in Pseudomonas putida DOT-T1E.”  Ramos J.L.et.al.   9642183
[2] “Three efflux pumps are required to provide efficient tolerance to toluene in Pseudomonas putida DOT-T1E.”  Rojas A.et.al.   11395460
[3] “Antibiotic-dependent induction of Pseudomonas putida DOT-T1E TtgABC efflux pump is mediated by the drug binding repressor TtgR.”  Teran W.et.al.   14506010

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MSKFFIDRPI FAWVIALVIM LVGALSILKL PINQYPSIAP PAIAIAVTYP GASAQTVQDT 
61:	VVQVIEQQLN GIDNLRYVSS ESNSDGSMTI TATFEQGTNP DTAQVQVQNK LNLATPLLPQ 
121:	EVQQQGIRVT KAVKNFLLVI GLVSEDGSMT KDDLANYIVS NMQDPISRTA GVGDFQVFGA 
181:	QYAMRIWLDP AKLNKFQLTP VDVKTAVAAQ NVQVSSGQLG GLPALPGTQL NATIIGKTRL 
241:	QTAEQFESIL LKVNKDGSQV RLGDVAQVGL GGENYAVSAQ FNGKPASGLA VKLATGANAL 
301:	DTAKALRETI KGLEPFFPPG VKAVFPYDTT PVVTESISGV IHTLIEAVVL VFLVMYLFLQ 
361:	NFRATIITTM TVPVVLLGTF GILAAAGFSI NTLTMFAMVL AIGLLVDDAI VVVENVERVM 
421:	SEEGLPPKEA TKRSMEQIQG ALVGIALVLS AVLLPMAFFG GSTGVIYRQF SITIVSAMGL 
481:	SVLVALIFTP ALCATMLKPL KKGEHHTAKG GFFGWFNRNF DRSVNGYERS VGTILRNKVP 
541:	FLLAYALIVV GMIWLFARIP TAFLPEEDQG VLFAQVQTPA GSSAERTQVV VDQMREYLLK 
601:	DEADTVSSVF TVNGFNFAGR GQSSGMAFIM LKPWDERSKE NSVFALAQRA QQHFFTFRDA 
661:	MVFAFAPPAV LELGNATGFD VFLQDRGGVG HAKLMEARNQ FLAKAAQSKI LSAVRPNGLN 
721:	DEPQYQLTID DERASALGVT IADINNTLSI ALGASYVNDF IDRGRVKKVY IQGEPSARMS 
781:	PEDLQKWYVR NGAGEMVPFS SFAKGEWTYG SPKLSRYNGV EAMEILGAPA PGYSTGEAMA 
841:	EVERIAGELP SGIGFSWTGM SYEEKLSGSQ MPALFALSVL FVFLCLAALY ESWSIPIAVV 
901:	LVVPLGIIGA LIATSLRGLS NDVYFLVGLL TTIGLAAKNA ILIVEFAKEL HEQGRSLYDA 
961:	AIEACRMRLR PIIMTSLAFI LGVVPLTIAS GAGAGSQHAI GTGVIGGMIS ATVLAIFWVP 
1021:	LFFVAVSSLF GSKEPEKDVT PENPRYEAGQ