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5.B.1.1.1
The gp91phox/p22phox NADPH oxidase-associated, cytochrome b558, Nox2. TMS2 is important for stability and electron transfer (Picciocchi et al., 2011). The integral membrane flavocytochrome of Nox 2 transfers an electron from intracellular NADPH to extracellular O2, generating superoxide anion, O2- (Fisher 2009).

Accession Number:P04839
Protein Name:C24B aka CYBB
Length:570
Molecular Weight:65336.00
Species:Homo sapiens (Human) [9606]
Number of TMSs:5
Location1 / Topology2 / Orientation3: Membrane1 / Multi-pass membrane protein2
Substrate Electron

Cross database links:

Genevestigator: P04839
eggNOG: prNOG08301
HEGENOM: HBG591219
RefSeq: NP_000388.2   
Entrez Gene ID: 1536   
Pfam: PF08022    PF01794    PF08030   
OMIM: 300481  gene
306400  phenotype
KEGG: hsa:1536   

Gene Ontology

GO:0043020 C:NADPH oxidase complex
GO:0009055 F:electron carrier activity
GO:0050660 F:FAD binding
GO:0020037 F:heme binding
GO:0046982 F:protein heterodimerization activity
GO:0016175 F:superoxide-generating NADPH oxidase activity
GO:0005244 F:voltage-gated ion channel activity
GO:0022900 P:electron transport chain
GO:0006954 P:inflammatory response
GO:0045087 P:innate immune response
GO:0006811 P:ion transport
GO:0045730 P:respiratory burst
GO:0042554 P:superoxide anion generation

References (26)

[1] “Cloning the gene for an inherited human disorder -- chronic granulomatous disease -- on the basis of its chromosomal location.”  Royer-Pokora B.et.al.   2425263
[2] “CYBB mutation analysis in X-linked chronic granulomatous disease.”  Jirapongsananuruk O.et.al.   12139950
[3] “Complete sequencing and characterization of 21,243 full-length human cDNAs.”  Ota T.et.al.   14702039
[4] “The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).”  The MGC Project Teamet.al.   15489334
[5] “The glycoprotein encoded by the X-linked chronic granulomatous disease locus is a component of the neutrophil cytochrome b complex.”  Dinauer M.C.et.al.   3600768
[6] “Nonhomologous recombination between the cytochrome b558 heavy chain gene (CYBB) and LINE-1 causes an X-linked chronic granulomatous disease.”  Kumatori A.et.al.   9790760
[7] “The X-linked chronic granulomatous disease gene codes for the beta-chain of cytochrome b-245.”  Teahan C.et.al.   3600769
[8] “Evidence that the product of the human X-linked CGD gene, gp91-phox, is a voltage-gated H(+) pathway.”  Henderson L.M.et.al.   10578014
[9] “Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry.”  Chen R.et.al.   19159218
[10] “A missense mutation in the neutrophil cytochrome b heavy chain in cytochrome-positive X-linked chronic granulomatous disease.”  Dinauer M.C.et.al.   2556453
[11] “Point mutations in the beta-subunit of cytochrome b558 leading to X-linked chronic granulomatous disease.”  Bolscher B.G.J.M.et.al.   1710153
[12] “A newly recognized point mutation in the cytochrome b558 heavy chain gene replacing alanine57 by glutamic acid, in a patient with cytochrome b positive X-linked chronic granulomatous disease.”  Ariga T.et.al.   8101486
[13] “Two novel point mutations in the cytochrome b 558 heavy chain gene, detected in two Japanese patients with X-linked chronic granulomatous disease.”  Ariga T.et.al.   7927345
[14] “A point mutation in gp91-phox of cytochrome b558 of the human NADPH oxidase leading to defective translocation of the cytosolic proteins p47-phox and p67-phox.”  Leusen J.H.W.et.al.   8182143
[15] “Identification of mutations in seven Chinese patients with X-linked chronic granulomatous disease.”  Hui Y.F.et.al.   8916969
[16] “An in-frame triplet deletion within the gp91-phox gene in an adult X-linked chronic granulomatous disease patient with residual NADPH-oxidase activity.”  Jendrossek V.et.al.   9111587
[17] “X-linked chronic granulomatous disease: mutations in the CYBB gene encoding the gp91-phox component of respiratory-burst oxidase.”  Rae J.et.al.   9585602
[18] “A novel mutation at a probable heme-binding ligand in neutrophil cytochrome b558 in atypical X-linked chronic granulomatous disease.”  Tsuda M.et.al.   9856476
[19] “Nicotinamide-adenine dinucleotide phosphate oxidase assembly and activation in EBV-transformed B lymphoblastoid cell lines of normal and chronic granulomatous disease patients.”  Dusi S.et.al.   9794433
[20] “Genetic analysis of 13 families with X-linked chronic granulomatous disease reveals a low proportion of sporadic patients and a high proportion of sporadic carriers.”  Ariga T.et.al.   9667376
[21] “Uncommon missense and splice mutations and resulting biochemical phenotypes in German patients with X-linked chronic granulomatous disease.”  Roesler J.et.al.   10089913
[22] “Molecular analysis of chronic granulomatous disease caused by defects in gp91-phox.”  Patino P.J.et.al.   9888386
[23] “Statistical and mutational analysis of chronic granulomatous disease in Japan with special reference to gp91-phox and p22-phox deficiency.”  Ishibashi F.et.al.   10914676
[24] “Characterization of 11 novel mutations in the X-linked chronic granulomatous disease (CYBB gene).”  Gerard B.et.al.   11462241
[25] “Molecular and functional characterization of a new X-linked chronic granulomatous disease variant (X91+) case with a double missense mutation in the cytosolic gp91phox C-terminal tail.”  Stasia M.J.et.al.   11997083
[26] “Functional analysis of two-amino acid substitutions in gp91 phox in a patient with X-linked flavocytochrome b558-positive chronic granulomatous disease by means of transgenic PLB-985 cells.”  Bionda C.et.al.   15338276
Structure:
3A1F     

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FASTA formatted sequence
1:	MGNWAVNEGL SIFVILVWLG LNVFLFVWYY RVYDIPPKFF YTRKLLGSAL ALARAPAACL 
61:	NFNCMLILLP VCRNLLSFLR GSSACCSTRV RRQLDRNLTF HKMVAWMIAL HSAIHTIAHL 
121:	FNVEWCVNAR VNNSDPYSVA LSELGDRQNE SYLNFARKRI KNPEGGLYLA VTLLAGITGV 
181:	VITLCLILII TSSTKTIRRS YFEVFWYTHH LFVIFFIGLA IHGAERIVRG QTAESLAVHN 
241:	ITVCEQKISE WGKIKECPIP QFAGNPPMTW KWIVGPMFLY LCERLVRFWR SQQKVVITKV 
301:	VTHPFKTIEL QMKKKGFKME VGQYIFVKCP KVSKLEWHPF TLTSAPEEDF FSIHIRIVGD 
361:	WTEGLFNACG CDKQEFQDAW KLPKIAVDGP FGTASEDVFS YEVVMLVGAG IGVTPFASIL 
421:	KSVWYKYCNN ATNLKLKKIY FYWLCRDTHA FEWFADLLQL LESQMQERNN AGFLSYNIYL 
481:	TGWDESQANH FAVHHDEEKD VITGLKQKTL YGRPNWDNEF KTIASQHPNT RIGVFLCGPE 
541:	ALAETLSKQS ISNSESGPRG VHFIFNKENF