5.A.2.1.2 The DsbI (223 aas and 4 TMSs in a 3 + 1 arrangement)/DsbL (222 aas and 1 N-terminal TMS) disulfide bond oxidoreductase/thio:disulfide interchange protein together form a dithiol oxidase system, acting on the periplasmic enzyme arylsulfate sulfotransferase, AssT (598 aas, 1 N-terminal TMS, Q8FDI4) which requires a disulfide bond for activity. The 3-d structure of DsbL at 1.55 Å resolution has been determined. DsbL is one of the most reactive oxidizing thioredoxin-like protein known (Grimshaw et al. 2008). These three enzymes are encoded within a tricistronic operon.
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Accession Number: | P0A4L7 |
Protein Name: | Thiol:disulfide interchange protein DsbL |
Length: | 222 |
Molecular Weight: | 24381.00 |
Species: | Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC) [199310] |
Number of TMSs: | 1 |
Location1 / Topology2 / Orientation3: |
Periplasm1 |
Substrate |
Electrons |
---|
1: MSKLGISSLF KTILLTAALA VSFTASAFTE GTDYMVLEKP IPNADKTLIK VFSYACPFCY
61: KYDKAVTGPV SEKVKDIVAF TPFHLETKGE YGKQASEVFA VLINKDKAAG ISLFDANSQF
121: KKAKFAYYAA YHDKKERWSD GKDPAAFIKT GLDAAGMSQA DFEAALKEPA VQETLEKWKA
181: SYDVAKIQGV PAYVVNGKYL IYTKSIKSID AMADLIRELA SK