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3.A.1.7.1
Phosphate porter, PhoSPstABC.  Serves as both a transporter and a sensor for transcriptional activation of the pho regulon in the presence of low external phosphate.  The unphosphorylated EIIANtr protein of the PTS (TC# 4.A) activates PhoR, the senor kinase that phosphorylates the response regulator, PhoB, that activates the pho regulon (Lüttmann et al. 2012).

Accession Number:P0AAH0
Protein Name:Phosphate import ATP-binding protein PstB aka PhoT aka B3725
Length:257
Molecular Weight:29027.00
Species:Escherichia coli [83333]
Location1 / Topology2 / Orientation3: Cell inner membrane1 / Peripheral membrane protein2
Substrate phosphate

Cross database links:

Genevestigator: P0AAH0
EchoBASE: EB0776
EcoGene: EG10783
eggNOG: COG1117
HEGENOM: HBG758042
DIP: DIP-47851N
RefSeq: AP_004062.1    NP_418181.1   
Entrez Gene ID: 948240   
Pfam: PF00005   
Drugbank: Drugbank Link   
BioCyc: EcoCyc:PSTB-MONOMER    ECOL168927:B3725-MONOMER   
KEGG: ecj:JW3703    eco:b3725   

Gene Ontology

GO:0005886 C:plasma membrane
GO:0005524 F:ATP binding
GO:0005315 F:inorganic phosphate transmembrane transport...
GO:0015415 F:phosphate transmembrane-transporting ATPase...
GO:0006817 P:phosphate transport

References (6)

[1] “Nucleotide sequence of the genes involved in phosphate transport and regulation of the phosphate regulon in Escherichia coli.”  Amemura M.et.al.   2993631
[2] “Phosphate-specific transport system of Escherichia coli: nucleotide sequence and gene-polypeptide relationships.”  Surin B.P.et.al.   3881386
[3] “DNA sequence and analysis of 136 kilobases of the Escherichia coli genome: organizational symmetry around the origin of replication.”  Burland V.D.et.al.   7686882
[4] “The complete genome sequence of Escherichia coli K-12.”  Blattner F.R.et.al.   9278503
[5] “Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.”  Hayashi K.et.al.   16738553
[6] “PstB protein of the phosphate-specific transport system of Escherichia coli is an ATPase.”  Chan F.-Y.et.al.   8682808

External Searches:

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  • 2° Structure (Network Protein Sequence Analysis)

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MSMVETAPSK IQVRNLNFYY GKFHALKNIN LDIAKNQVTA FIGPSGCGKS TLLRTFNKMF 
61:	ELYPEQRAEG EILLDGDNIL TNSQDIALLR AKVGMVFQKP TPFPMSIYDN IAFGVRLFEK 
121:	LSRADMDERV QWALTKAALW NETKDKLHQS GYSLSGGQQQ RLCIARGIAI RPEVLLLDEP 
181:	CSALDPISTG RIEELITELK QDYTVVIVTH NMQQAARCSD HTAFMYLGEL IEFSNTDDLF 
241:	TKPAKKQTED YITGRYG