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3.A.1.12.1
Glycine betaine/proline porter, ProU or ProVWX (also transports proline betaine, carnitine, dimethyl proline, homobetaine, γ-butyrobetaine and choline with low affinity).  Contributes to the regulation of cell volume is response to osmolarity.  A reconsituted system shows osmotic strength-gating (Gul and Poolman 2012).

Accession Number:P0AFM2
Protein Name:ProX aka ProU aka B2679
Length:330
Molecular Weight:36023.00
Species:Escherichia coli [83333]
Location1 / Topology2 / Orientation3: Periplasm1
Substrate Gamma-butyrobetaine, Homobetaine, Dimethylproline, Proline, Glycine betaine, Carnitine, Choline

Cross database links:

Genevestigator: P0AFM2
EchoBASE: EB0766
EcoGene: EG10773
eggNOG: COG2113
HEGENOM: HBG298949
RefSeq: AP_003252.1    NP_417165.1   
Entrez Gene ID: 947165   
Pfam: PF04069   
Drugbank: Drugbank Link   
BioCyc: EcoCyc:PROX-MONOMER    ECOL168927:B2679-MONOMER   
KEGG: ecj:JW2654    eco:b2679   

Gene Ontology

GO:0042597 C:periplasmic space
GO:0005515 F:protein binding
GO:0005215 F:transporter activity
GO:0006865 P:amino acid transport

References (6)

[1] “Nucleotide sequence of the osmoregulatory proU operon of Escherichia coli.”  Gowrishankar J.et.al.   2649479
[2] “Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features.”  Yamamoto Y.et.al.   9205837
[3] “The complete genome sequence of Escherichia coli K-12.”  Blattner F.R.et.al.   9278503
[4] “Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.”  Hayashi K.et.al.   16738553
[5] “Purification and characterization of a glycine betaine binding protein from Escherichia coli.”  Barron A.et.al.   3305496
[6] “Cation-pi interactions as determinants for binding of the compatible solutes glycine betaine and proline betaine by the periplasmic ligand-binding protein ProX from Escherichia coli.”  Schiefner A.et.al.   14612446
Structure:
1R9L   1R9Q     

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MRHSVLFATA FATLISTQTF AADLPGKGIT VNPVQSTITE ETFQTLLVSR ALEKLGYTVN 
61:	KPSEVDYNVG YTSLASGDAT FTAVNWTPLH DNMYEAAGGD KKFYREGVFV NGAAQGYLID 
121:	KKTADQYKIT NIAQLKDPKI AKLFDTNGDG KADLTGCNPG WGCEGAINHQ LAAYELTNTV 
181:	THNQGNYAAM MADTISRYKE GKPVFYYTWT PYWVSNELKP GKDVVWLQVP FSALPGDKNA 
241:	DTKLPNGANY GFPVSTMHIV ANKAWAEKNP AAAKLFAIMQ LPVADINAQN AIMHDGKASE 
301:	GDIQGHVDGW IKAHQQQFDG WVNEALAAQK