TCDB is operated by the Saier Lab Bioinformatics Group
« See all members of the family


3.A.1.5.21
The Microcin C/peptide uptake porter, YejABEF (Novikova et al., 2007).  The 'Trojan horse' antibiotic, microcin C, consists of a nonhydrolyzable aspartyl-adenylate that is efficiently imported into bacterial cells owing to a covalently attached peptide. Once inside the cell, the peptide "carrier" is removed by proteolytic processing to release a potent aspartyl tRNA synthetase inhibitor (Severinov and Nair 2012).

Accession Number:P0AFU1
Protein Name:YejB
Length:364
Molecular Weight:40360.00
Species:Escherichia coli O157:H7 [83334]
Number of TMSs:6
Location1 / Topology2 / Orientation3: Cell inner membrane1 / Multi-pass membrane protein2
Substrate Microcin C

Cross database links:

HEGENOM: HBG724229
RefSeq: NP_288761.1    NP_311097.1   
Entrez Gene ID: 916774    957359   
Pfam: PF00528   
BioCyc: ECOL83334:ECS3070-MONOMER   
KEGG: ece:Z3437    ecs:ECs3070   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0005215 F:transporter activity
GO:0006810 P:transport

References (2)

[1] “Genome sequence of enterohaemorrhagic Escherichia coli O157:H7.”  Perna N.T.et.al.   11206551
[2] “Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12.”  Hayashi T.et.al.   11258796

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
Window Size: Angle:  
Window Size: Angle:  
FASTA formatted sequence
1:	MGAYLIRRLL LVIPTLWAII TINFFIVQIA PGGPVDQAIA AIEFGNAGVL PGAGGEGVRA 
61:	SHAQTGVGNI SDSNYRGGRG LDPEVIAEIT HRYGFDKPIH ERYFKMLWDY IRFDFGDSLF 
121:	RSASVLTLIK DSLPVSITLG LWSTLIIYLV SIPLGIRKAV YNGSRFDVWS SAFIIIGYAI 
181:	PAFLFAILLI VFFAGGSYFD LFPLRGLVSA NFDSLPWYQK ITDYLWHITL PVLATVIGGF 
241:	AALTMLTKNS FLDEVRKQYV VTARAKGVSE KNILWKHVFR NAMLLVIAGF PATFISMFFT 
301:	GSLLIEVMFS LNGLGLLGYE ATVSRDYPVM FGTLYIFTLI GLLLNIVSDI SYTLVDPRID 
361:	FEGR