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3.A.1.12.1
Glycine betaine/proline porter, ProU or ProVWX (also transports proline betaine, carnitine, dimethyl proline, homobetaine, γ-butyrobetaine and choline with low affinity).  Contributes to the regulation of cell volume is response to osmolarity.  A reconsituted system shows osmotic strength-gating (Gul and Poolman 2012).

Accession Number:P14175
Protein Name:ProV aka B2677
Length:400
Molecular Weight:44163.00
Species:Escherichia coli [83333]
Number of TMSs:1
Location1 / Topology2 / Orientation3: Cell inner membrane1 / Peripheral membrane protein2
Substrate Gamma-butyrobetaine, Homobetaine, Dimethylproline, Proline, Glycine betaine, Carnitine, Choline

Cross database links:

Genevestigator: P14175
EchoBASE: EB0764
EcoGene: EG10771
eggNOG: COG4175
HEGENOM: HBG758042
DIP: DIP-10574N
RefSeq: AP_003250.1    NP_417163.1   
Entrez Gene ID: 947148   
Pfam: PF00005    PF00571   
Drugbank: Drugbank Link   
BioCyc: EcoCyc:PROV-MONOMER    ECOL168927:B2677-MONOMER   
KEGG: ecj:JW2652    eco:b2677   

Gene Ontology

GO:0005886 C:plasma membrane
GO:0015171 F:amino acid transmembrane transporter activity
GO:0005524 F:ATP binding
GO:0016887 F:ATPase activity
GO:0005515 F:protein binding
GO:0006865 P:amino acid transport

References (6)

[1] “Nucleotide sequence of the osmoregulatory proU operon of Escherichia coli.”  Gowrishankar J.et.al.   2649479
[2] “Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features.”  Yamamoto Y.et.al.   9205837
[3] “The complete genome sequence of Escherichia coli K-12.”  Blattner F.R.et.al.   9278503
[4] “Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.”  Hayashi K.et.al.   16738553
[5] “Molecular characterization of the proU loci of Salmonella typhimurium and Escherichia coli encoding osmoregulated glycine betaine transport systems.”  Stirling D.A.et.al.   2691838
[6] “Protein complexes of the Escherichia coli cell envelope.”  Stenberg F.et.al.   16079137

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MAIKLEIKNL YKIFGEHPQR AFKYIEQGLS KEQILEKTGL SLGVKDASLA IEEGEIFVIM 
61:	GLSGSGKSTM VRLLNRLIEP TRGQVLIDGV DIAKISDAEL REVRRKKIAM VFQSFALMPH 
121:	MTVLDNTAFG MELAGINAEE RREKALDALR QVGLENYAHS YPDELSGGMR QRVGLARALA 
181:	INPDILLMDE AFSALDPLIR TEMQDELVKL QAKHQRTIVF ISHDLDEAMR IGDRIAIMQN 
241:	GEVVQVGTPD EILNNPANDY VRTFFRGVDI SQVFSAKDIA RRTPNGLIRK TPGFGPRSAL 
301:	KLLQDEDREY GYVIERGNKF VGAVSIDSLK TALTQQQGLD AALIDAPLAV DAQTPLSELL 
361:	SHVGQAPCAV PVVDEDQQYV GIISKGMLLR ALDREGVNNG