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3.A.1.12.1
Glycine betaine/proline porter, ProU or ProVWX (also transports proline betaine, carnitine, dimethyl proline, homobetaine, γ-butyrobetaine and choline with low affinity).  Contributes to the regulation of cell volume is response to osmolarity.  A reconsituted system shows osmotic strength-gating (Gul and Poolman 2012).

Accession Number:P14176
Protein Name:ProW aka B2678
Length:354
Molecular Weight:37620.00
Species:Escherichia coli [83333]
Number of TMSs:7
Location1 / Topology2 / Orientation3: Cell inner membrane1 / Multi-pass membrane protein2
Substrate Gamma-butyrobetaine, Homobetaine, Dimethylproline, Proline, Glycine betaine, Carnitine, Choline

Cross database links:

Genevestigator: P14176
EchoBASE: EB0765
EcoGene: EG10772
eggNOG: COG4176
HEGENOM: HBG592565
RefSeq: AP_003251.1    NP_417164.1   
Entrez Gene ID: 947145   
Pfam: PF00528   
Drugbank: Drugbank Link   
BioCyc: EcoCyc:PROW-MONOMER    ECOL168927:B2678-MONOMER   
KEGG: ecj:JW2653    eco:b2678   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0005215 F:transporter activity
GO:0006865 P:amino acid transport

References (5)

[1] “Nucleotide sequence of the osmoregulatory proU operon of Escherichia coli.”  Gowrishankar J.et.al.   2649479
[2] “Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features.”  Yamamoto Y.et.al.   9205837
[3] “The complete genome sequence of Escherichia coli K-12.”  Blattner F.R.et.al.   9278503
[4] “Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.”  Hayashi K.et.al.   16738553
[5] “Global topology analysis of the Escherichia coli inner membrane proteome.”  Daley D.O.et.al.   15919996

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
Window Size: Angle:  
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FASTA formatted sequence
1:	MADQNNPWDT TPAADSAAQS ADAWGTPTTA PTDGGGADWL TSTPAPNVEH FNILDPFHKT 
61:	LIPLDSWVTE GIDWVVTHFR PVFQGVRVPV DYILNGFQQL LLGMPAPVAI IVFALIAWQI 
121:	SGVGMGVATL VSLIAIGAIG AWSQAMVTLA LVLTALLFCI VIGLPLGIWL ARSPRAAKII 
181:	RPLLDAMQTT PAFVYLVPIV MLFGIGNVPG VVVTIIFALP PIIRLTILGI NQVPADLIEA 
241:	SRSFGASPRQ MLFKVQLPLA MPTIMAGVNQ TLMLALSMVV IASMIAVGGL GQMVLRGIGR 
301:	LDMGLATVGG VGIVILAIIL DRLTQAVGRD SRSRGNRRWY TTGPVGLLTR PFIK