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1.A.6.2.2
Touch-responsive mechanosensitive degenerin channel complex (Mec-4/Mec-10 form the cation/Ca2+-permeable channel; Mec-2 and Mec-6 regulate) (Bianchi, 2007; Chelur et al., 2002; ). Mec-6 is a chaparone protein required for functional insertion (Matthewman et al. 2018). Mec-10 plays a role in the response to mechanical forces such as laminar shear stress (Shi et al. 2016). MEC-4 or MEC-10 mutants that alter the channel's LSS response are primarily clustered between the degenerin site and the selectivity filter, a region that likely forms the narrowest portion of the channel pore (Shi et al. 2018). TMS2 forms the Ca2+ channel of Mec-4.  A C-terminal domain affects trafficking of a neuronally expressed DEG/ENaC. Neuronal swelling occurs prior to commitment to necrotic death (Royal et al. 2005).

Accession Number:P24612
Protein Name:Mec-4
Length:768
Molecular Weight:87194.00
Species:Caenorhabditis elegans [6239]
Number of TMSs:3
Location1 / Topology2 / Orientation3: Membrane1 / Multi-pass membrane protein2
Substrate Na+

Cross database links:

eggNOG: meNOG13575
HEGENOM: HBG388583
RefSeq: NP_510712.2   
Entrez Gene ID: 181728   
Pfam: PF00858   
KEGG: cel:T01C8.7   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005272 F:sodium channel activity
GO:0008219 P:cell death
GO:0006814 P:sodium ion transport

References (4)

[1] “Sequence and transmembrane topology of MEC-4, an ion channel subunit required for mechanotransduction in Caenorhabditis elegans.”  Lai C.C.et.al.   8655580
[2] “Genome sequence of the nematode C. elegans: a platform for investigating biology.”  The C. elegans sequencing consortiumet.al.   9851916
[3] “The mec-4 gene is a member of a family of Caenorhabditis elegans genes that can mutate to induce neuronal degeneration.”  Driscoll M.et.al.   1672038
[4] “The mechanosensory protein MEC-6 is a subunit of the C. elegans touch-cell degenerin channel.”  Chelur D.S.et.al.   12478294
Structure:
2K2B     

External Searches:

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  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MSWMQNLKNY QHLRDPSEYM SQVYGDPLAY LQETTKFVTE REYYEDFGYG ECFNSTESEV 
61:	QCELITGEFD PKLLPYDKRL AWHFKEFCYK TSAHGIPMIG EAPNVYYRAV WVVLFLGCMI 
121:	MLYLNAQSVL DKYNRNEKIV DIQLKFDTAP FPAITLCNLN PYKASLATSV DLVKRTLSAF 
181:	DGAMGKAGGN KDHEEEREVV TEPPTTPAPT TKPARRRGKR DLSGAFFEPG FARCLCGSQG 
241:	SSEQEDKDEE KEEELLETTT KKVFNINDAD EEWDGMEEYD NEHYENYDVE ATTGMNMMEE 
301:	CQSERTKFDE PTGFDDRCIC AFDRSTHDAW PCFLNGTWET TECDTCNEHA FCTKDNKTAK 
361:	GHRSPCICAP SRFCVAYNGK TPPIEIWTYL QGGTPTEDPN FLEAMGFQGM TDEVAIVTKA 
421:	KENIMFAMAT LSMQDRERLS TTKRELVHKC SFNGKACDIE ADFLTHIDPA FGSCFTFNHN 
481:	RTVNLTSIRA GPMYGLRMLV YVNASDYMPT TEATGVRLTI HDKEDFPFPD TFGYSAPTGY 
541:	VSSFGLRLRK MSRLPAPYGD CVPDGKTSDY IYSNYEYSVE GCYRSCFQQL VLKECRCGDP 
601:	RFPVPENARH CDAADPIARK CLDARMNDLG GLHGSFRCRC QQPCRQSIYS VTYSPAKWPS 
661:	LSLQIQLGSC NGTAVECNKH YKENGAMVEV FYEQLNFEML TESEAYGFVN LLADFGGQLG 
721:	LWCGISFLTC CEFVFLFLET AYMSAEHNYS LYKKKKAEKA KKIASGSF