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3.A.1.5.21
The Microcin C/peptide uptake porter, YejABEF (Novikova et al., 2007).  The 'Trojan horse' antibiotic, microcin C, consists of a nonhydrolyzable aspartyl-adenylate that is efficiently imported into bacterial cells owing to a covalently attached peptide. Once inside the cell, the peptide "carrier" is removed by proteolytic processing to release a potent aspartyl tRNA synthetase inhibitor (Severinov and Nair 2012).

Accession Number:P33913
Protein Name:YejA
Length:604
Molecular Weight:69669.00
Species:Escherichia coli (strain K12) [83333]
Number of TMSs:1
Location1 / Topology2 / Orientation3: Cell outer membrane1
Substrate Microcin C

Cross database links:

Genevestigator: P33913
EchoBASE: EB1972
EcoGene: EG12037
eggNOG: COG4166
HEGENOM: HBG289570
RefSeq: AP_002775.1    NP_416682.4   
Entrez Gene ID: 946675   
Pfam: PF00496   
BioCyc: EcoCyc:YEJA-MONOMER    ECOL168927:B2177-MONOMER   
KEGG: ecj:JW2165    eco:b2177   

Gene Ontology

GO:0005215 F:transporter activity
GO:0042884 P:microcin transport

References (3)

[1] “The complete genome sequence of Escherichia coli K-12.”  Blattner F.R.et.al.   9278503
[2] “Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.”  Hayashi K.et.al.   16738553
[3] “A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map.”  Itoh T.et.al.   9097040

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MIVRILLLFI ALFTFGVQAQ AIKESYAFAV LGEPRYAFNF NHFDYVNPAA PKGGQITLSA 
61:	LGTFDNFNRY ALRGNPGART EQLYDTLFTT SDDEPGSYYP LIAESARYAD DYSWVEVAIN 
121:	PRARFHDGSP ITARDVEFTF QKFMTEGVPQ FRLVYKGTTV KAIAPLTVRI ELAKPGKEDM 
181:	LSLFSLPVFP EKYWKDHKLS DPLATPPLAS GPYRVTSWKM GQNIVYSRVK DYWAANLPVN 
241:	RGRWNFDTIR YDYYLDDNVA FEAFKAGAFD LRMENDAKNW ATRYTGKNFD KKYIIKDEQK 
301:	NESAQDTRWL AFNIQRPVFS DRRVREAITL AFDFEWMNKA LFYNAWSRTN SYFQNTEYAA 
361:	RNYPDAAELV LLAPMKKDLP SEVFTQIYQP PVSKGDGYDR DNLLKADKLL NEAGWVLKGQ 
421:	QRVNATTGQP LSFELLLPAS SNSQWVLPFQ HSLQRLGINM DIRKVDNSQI TNRMRSRDYD 
481:	MMPRVWRAMP WPSSDLQISW SSEYINSTYN APGVQSPVID SLINQIIAAQ GNKEKLLPLG 
541:	RALDRVLTWN YYMLPMWYMA EDRLAWWDKF SQPAVRPIYS LGIDTWWYDV NKAAKLPSAS 
601:	KQGE