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3.A.1.5.21
The Microcin C/peptide uptake porter, YejABEF (Novikova et al., 2007).  The 'Trojan horse' antibiotic, microcin C, consists of a nonhydrolyzable aspartyl-adenylate that is efficiently imported into bacterial cells owing to a covalently attached peptide. Once inside the cell, the peptide "carrier" is removed by proteolytic processing to release a potent aspartyl tRNA synthetase inhibitor (Severinov and Nair 2012).

Accession Number:P33915
Protein Name:YejE
Length:341
Molecular Weight:38092.00
Species:Escherichia coli (strain K12) [83333]
Number of TMSs:6
Location1 / Topology2 / Orientation3: Cell inner membrane1 / Multi-pass membrane protein2
Substrate Microcin C

Cross database links:

Genevestigator: P33915
EchoBASE: EB1974
EcoGene: EG12040
eggNOG: COG4239
HEGENOM: HBG729429
DIP: DIP-11935N
RefSeq: AP_002777.1    NP_416684.1   
Entrez Gene ID: 946683   
Pfam: PF00528   
BioCyc: EcoCyc:YEJE-MONOMER    ECOL168927:B2179-MONOMER   
KEGG: ecj:JW2167    eco:b2179   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0005215 F:transporter activity
GO:0042884 P:microcin transport

References (4)

[1] “A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map.”  Itoh T.et.al.   9097040
[2] “The complete genome sequence of Escherichia coli K-12.”  Blattner F.R.et.al.   9278503
[3] “Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.”  Hayashi K.et.al.   16738553
[4] “Global topology analysis of the Escherichia coli inner membrane proteome.”  Daley D.O.et.al.   15919996

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MSRLSPVNQA RWARFRHNRR GYWSLWIFLV LFGLSLCSEL IANDKPLLVR YDGSWYFPLL 
61:	KNYSESDFGG PLASQADYQD PWLKQRLENN GWVLWAPIRF GATSINFATN KPFPSPPSRQ 
121:	NWLGTDANGG DVLARILYGT RISVLFGLML TLCSSVMGVL AGALQGYYGG KVDLWGQRFI 
181:	EVWSGMPTLF LIILLSSVVQ PNFWWLLAIT VLFGWMSLVG VVRAEFLRTR NFDYIRAAQA 
241:	LGVSDRSIIL RHMLPNAMVA TLTFLPFILC SSITTLTSLD FLGFGLPLGS PSLGELLLQG 
301:	KNNLQAPWLG ITAFLSVAIL LSLLIFIGEA VRDAFDPNKA V