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3.A.1.5.21
The Microcin C/peptide uptake porter, YejABEF (Novikova et al., 2007).  The 'Trojan horse' antibiotic, microcin C, consists of a nonhydrolyzable aspartyl-adenylate that is efficiently imported into bacterial cells owing to a covalently attached peptide. Once inside the cell, the peptide "carrier" is removed by proteolytic processing to release a potent aspartyl tRNA synthetase inhibitor (Severinov and Nair 2012).

Accession Number:P33916
Protein Name:YejF
Length:529
Molecular Weight:58726.00
Species:Escherichia coli (strain K12) [83333]
Location1 / Topology2 / Orientation3: Cell inner membrane1 / Multi-pass membrane protein2
Substrate Microcin C

Cross database links:

Genevestigator: P33916
EchoBASE: EB1975
EcoGene: EG12041
eggNOG: COG4172
HEGENOM: HBG758042
DIP: DIP-11936N
RefSeq: AP_002778.1    NP_416685.1   
Entrez Gene ID: 946689   
Pfam: PF00005    PF08352   
BioCyc: EcoCyc:YEJF-MONOMER    ECOL168927:B2180-MONOMER   
KEGG: ecj:JW2168    eco:b2180   

Gene Ontology

GO:0005524 F:ATP binding
GO:0016887 F:ATPase activity
GO:0042884 P:microcin transport

References (3)

[1] “The complete genome sequence of Escherichia coli K-12.”  Blattner F.R.et.al.   9278503
[2] “Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.”  Hayashi K.et.al.   16738553
[3] “A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map.”  Itoh T.et.al.   9097040

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MTQTLLAIEN LSVGFRHQQT VRTVVNDVSL QIEAGETLAL VGESGSGKSV TALSILRLLP 
61:	SPPVEYLSGD IRFHGESLLH ASDQTLRGVR GNKIAMIFQE PMVSLNPLHT LEKQLYEVLS 
121:	LHRGMRREAA RGEILNCLDR VGIRQAAKRL TDYPHQLSGG ERQRVMIAMA LLTRPELLIA 
181:	DEPTTALDVS VQAQILQLLR ELQGELNMGM LFITHNLSIV RKLAHRVAVM QNGRCVEQNY 
241:	AATLFASPTH PYTQKLLNSE PSGDPVPLPE PASTLLDVEQ LQVAFPIRKG ILKRIVDHNV 
301:	VVKNISFTLR AGETLGLVGE SGSGKSTTGL ALLRLINSQG SIIFDGQPLQ NLNRRQLLPI 
361:	RHRIQVVFQD PNSSLNPRLN VLQIIEEGLR VHQPTLSAAQ REQQVIAVMH EVGLDPETRH 
421:	RYPAEFSGGQ RQRIAIARAL ILKPSLIILD EPTSSLDKTV QAQILTLLKS LQQKHQLAYL 
481:	FISHDLHVVR ALCHQVIILR QGEVVEQGPC ARVFATPQQE YTRQLLALS