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3.A.11.1.1
DNA translocase. The three-layered DNA uptake machinery at the cell pole in competent Bacillus subtilis cells is a stable complex (Kaufenstein et al., 2011).

Accession Number:P39145
Protein Name:CMF1 aka ComFA aka ComF1
Length:463
Molecular Weight:52566.00
Species:Bacillus subtilis [1423]
Location1 / Topology2 / Orientation3: Cell membrane1 / Multi-pass membrane protein2
Substrate ssDNA

Cross database links:

HEGENOM: HBG535566
RefSeq: NP_391427.1   
Entrez Gene ID: 938537   
Pfam: PF00271    PF04851   
KEGG: bsu:BSU35470   

Gene Ontology

GO:0005524 F:ATP binding
GO:0003677 F:DNA binding
GO:0004386 F:helicase activity
GO:0030420 P:establishment of competence for transformation

References (3)

[1] “comF, a Bacillus subtilis late competence locus, encodes a protein similar to ATP-dependent RNA/DNA helicases.”  Londono-Vallejo J.A.et.al.   8412657
[2] “Sequence of the 305 degrees-307 degrees region of the Bacillus subtilis chromosome.”  Soldo B.et.al.   8969505
[3] “The complete genome sequence of the Gram-positive bacterium Bacillus subtilis.”  Kunst F.et.al.   9384377

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MNVPVEKNSS FSKELQQTLR SRHLLRTELS FSDEMIEWHI KNGYITAENS ISINKRRYRC 
61:	NRCGQTDQRY FSFYHSSGKN KLYCRSCVMM GRVSEEVPLY SWKEENESNW KSIKLTWDGK 
121:	LSSGQQKAAN VLIEAISKKE ELLIWAVCGA GKTEMLFPGI ESALNQGLRV CIATPRTDVV 
181:	LELAPRLKAA FQGADISALY GGSDDKGRLS PLMISTTHQL LRYKDAIDVM IIDEVDAFPY 
241:	SADQTLQFAV QKARKKNSTL VYLSATPPKE LKRKALNGQL HSVRIPARHH RKPLPEPRFV 
301:	WCGNWKKKLN RNKIPPAVKR WIEFHVKEGR PVFLFVPSVS ILEKAAACFK GVHCRTASVH 
361:	AEDKHRKEKV QQFRDGQLDL LITTTILERG VTVPKVQTGV LGAESSIFTE SALVQIAGRT 
421:	GRHKEYADGD VIYFHFGKTK SMLDARKHIK EMNELAAKVE CTD