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4.A.7.1.1
The L-ascorbate transporting and phosphorylating group translocator, SgaTBA or UlaCBA (SgaT = UlaA = YjfS; SgaB = UlaB = YjfT; SgaA = UlaC = PtxA = YjfU) (Hvorup et al., 2003; Zhang et al., 2003). Two conformations of the 3-d structure have been determined at 1.65 and 2.35 Å resolution, respectively (Luo et al., 2015). UlaA (SgaT) forms a homodimer with a novel fold. Each UlaA protomer consists of 11 TMSs arranged into a 'V-motif' domain and a 'core' domain. The V motifs form the interface between the two protomers, and the core-domain residues coordinate vitamin C. Alternating access of the substrate to the two sides of the cell membrane may be achieved through rigid-body rotation of the core relative to the V motif (Luo et al., 2015; Zhang et al., 2003). This structure does not resemble the ChbC structure (TC# 4.A.3.2.8).

Accession Number:P39301
Protein Name:SgaT aka B4193
Length:465
Molecular Weight:50737.00
Species:Escherichia coli [83333]
Number of TMSs:11
Location1 / Topology2 / Orientation3: Cell inner membrane1 / Multi-pass membrane protein2
Substrate Ascorbate

Cross database links:

Genevestigator: P39301
EchoBASE: EB2386
EcoGene: EG12493
eggNOG: COG3037
HEGENOM: HBG296693
DIP: DIP-10870N
RefSeq: AP_004693.1    NP_418614.4   
Entrez Gene ID: 948717   
Pfam: PF03611   
BioCyc: EcoCyc:SGAT-MONOMER    ECOL168927:B4193-MONOMER   
KEGG: ecj:JW5744    eco:b4193   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0008982 F:protein-N(PI)-phosphohistidine-sugar phosph...
GO:0009401 P:phosphoenolpyruvate-dependent sugar phospho...

References (7)

[1] “Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes.”  Burland V.D.et.al.   7610040
[2] “The complete genome sequence of Escherichia coli K-12.”  Blattner F.R.et.al.   9278503
[3] “Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.”  Hayashi K.et.al.   16738553
[4] “The gene yjfQ encodes the repressor of the yjfR-X regulon (ula), which is involved in L-ascorbate metabolism in Escherichia coli.”  Campos E.et.al.   12374842
[5] “The ascorbate transporter of Escherichia coli.”  Zhang Z.et.al.   12644495
[6] “Regulation of expression of the divergent ulaG and ulaABCDEF operons involved in L-ascorbate dissimilation in Escherichia coli.”  Campos E.et.al.   14996803
[7] “Global topology analysis of the Escherichia coli inner membrane proteome.”  Daley D.O.et.al.   15919996
Structure:
4RP8   4RP9     

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Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MEILYNIFTV FFNQVMTNAP LLLGIVTCLG YILLRKSVSV IIKGTIKTII GFMLLQAGSG 
61:	ILTSTFKPVV AKMSEVYGIN GAISDTYASM MATIDRMGDA YSWVGYAVLL ALALNICYVL 
121:	LRRITGIRTI MLTGHIMFQQ AGLIAVTLFI FGYSMWTTII CTAILVSLYW GITSNMMYKP 
181:	TQEVTDGCGF SIGHQQQFAS WIAYKVAPFL GKKEESVEDL KLPGWLNIFH DNIVSTAIVM 
241:	TIFFGAILLS FGIDTVQAMA GKVHWTVYIL QTGFSFAVAI FIITQGVRMF VAELSEAFNG 
301:	ISQRLIPGAV LAIDCAAIYS FAPNAVVWGF MWGTIGQLIA VGILVACGSS ILIIPGFIPM 
361:	FFSNATIGVF ANHFGGWRAA LKICLVMGMI EIFGCVWAVK LTGMSAWMGM ADWSILAPPM 
421:	MQGFFSIGIA FMAVIIVIAL AYMFFAGRAL RAEEDAEKQL AEQSA