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3.A.11.1.1
DNA translocase. The three-layered DNA uptake machinery at the cell pole in competent Bacillus subtilis cells is a stable complex (Kaufenstein et al., 2011).

Accession Number:P39694
Protein Name:CME1 aka ComEA aka ComE1
Length:205
Molecular Weight:21769.00
Species:Bacillus subtilis [1423]
Number of TMSs:1
Location1 / Topology2 / Orientation3: Cell membrane1 / Single-pass type II membrane protein2
Substrate ssDNA

Cross database links:

HEGENOM: HBG728721
RefSeq: NP_390437.1   
Entrez Gene ID: 937827   
Pfam: PF00633    PF10531   
KEGG: bsu:BSU25590   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0003677 F:DNA binding
GO:0006281 P:DNA repair
GO:0030420 P:establishment of competence for transformation

References (4)

[1] “Characterization of comE, a late competence operon of Bacillus subtilis required for the binding and uptake of transforming DNA.”  Hahn J.et.al.   7968523
[2] “Systematic sequencing of the 283 kb 210 degrees-232 degrees region of the Bacillus subtilis genome containing the skin element and many sporulation genes.”  Mizuno M.et.al.   8969508
[3] “The complete genome sequence of the Gram-positive bacterium Bacillus subtilis.”  Kunst F.et.al.   9384377
[4] “ComEA, a Bacillus subtilis integral membrane protein required for genetic transformation, is needed for both DNA binding and transport.”  Inamine G.S.et.al.   7768800

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MNWLNQHKKA IILAASAAVF TAIMIFLATG KNKEPVKQAV PTETENTVVK QEANNDESNE 
61:	TIVIDIKGAV QHPGVYEMRT GDRVSQAIEK AGGTSEQADE AQVNLAEILQ DGTVVYIPKK 
121:	GEETAVQQGG GGSVQSDGGK GALVNINTAT LEELQGISGV GPSKAEAIIA YREENGRFQT 
181:	IEDITKVSGI GEKSFEKIKS SITVK