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3.A.11.1.1
DNA translocase. The three-layered DNA uptake machinery at the cell pole in competent Bacillus subtilis cells is a stable complex (Kaufenstein et al., 2011).

Accession Number:P39695
Protein Name:CME3 aka ComEC aka ComE3
Length:776
Molecular Weight:86701.00
Species:Bacillus subtilis [1423]
Number of TMSs:12
Location1 / Topology2 / Orientation3: Cell membrane1 / Multi-pass membrane protein2
Substrate ssDNA

Cross database links:

HEGENOM: HBG314146
RefSeq: NP_390435.1   
Entrez Gene ID: 937839   
Pfam: PF03772   
KEGG: bsu:BSU25570   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0016787 F:hydrolase activity
GO:0030420 P:establishment of competence for transformation
GO:0006810 P:transport

References (3)

[1] “Characterization of comE, a late competence operon of Bacillus subtilis required for the binding and uptake of transforming DNA.”  Hahn J.et.al.   7968523
[2] “Systematic sequencing of the 283 kb 210 degrees-232 degrees region of the Bacillus subtilis genome containing the skin element and many sporulation genes.”  Mizuno M.et.al.   8969508
[3] “The complete genome sequence of the Gram-positive bacterium Bacillus subtilis.”  Kunst F.et.al.   9384377

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MRNSRLLLPM AAASATAGIT AAAYFPAIFL FILFLLIILI KTRHAFLIIV CFFSFILFFV 
61:	LYAVTDSQNV SSYRQGTYQF KAVIDTIPKI DGDRMSMMVE TPDKEKWAAA YRIQSAGEKE 
121:	QLLYIEPGMS CELTGTLEEP NHATVPGAFD YNEYLYRQHI HWNYSVTSIQ NCSEPENFKY 
181:	KVLSLRKHII SFTNSLLPPD STGIVQALTV GDRFYVEDEV LTAYQKLGVV HLLAISGLHV 
241:	GILTAGLFYI MIRLGITREK ASILLLLFLP LYVMLTGAAP SVLRAALMSG VYLAGSLVKW 
301:	RVRSATAICL SYIVLLLFNP YHLFEAGFQL SFAVSFSLIL SSSIFQQVKT SLGQLTIVSL 
361:	IAQLGSLPIL LYHFHQFSII SVPMNMLMVP FYTFCILPGA VAGVLLLSLS ASFGRLFFSW 
421:	FDLLISWINR LITNIADVDV FTIMIAHPAP VLLFLFTVTI ILLLMAIEKR SLSQLMVTGG 
481:	ICCTVMFLLF IYPCLSSEGE VDMIDIGQGD SMFVGAPHQR GRVLIDTGGT LSYSSEPWRE 
541:	KQHPFSLGEK VLIPFLTAKG IKQLDALILT HADQDHIGEA EILLKHHKVK RLVIPKGFVS 
601:	EPKDEKVLQA AREEGVAIEE VKRGDVLQIK DLQFHVLSPE APDPASKNNS SLVLWMETGG 
661:	MSWILTGDLE KEGEQEVMNV FPNIKADVLK VGHHGSKGST GEEFIQQLQP KTAIISAGKN 
721:	NRYHHPHQKV LQLLQRHSIR VLRTDQNGTI QYRYKNRVGT FSVYPPYDTS DITETN