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4.A.2.1.5
The mannitol porter (MtlA) (mannitol-1-P forming), MtlAF.  The system is encoded by the mannitol catabolic operon, mtlAFD, and is regulated by the transcription factor, MtlR (Joyet et al. 2015). MtlR contains an N-terminal helix-turn-helix motif followed by an Mga-like domain, two PTS regulatory domains (PRDs), an Enzyme IIBGat-like domain and an Enzyme IIAMtl-like domain, the last four of which can be phosphorylated by the PTS.  MtlR proteins are also found in Geobacillus stearothermophilus and Lactobacillus casei, but the mechanisms of their action are different (Joyet et al. 2015).  The dephosphorlyated form of the protein activates transcription (Heravi and Altenbuchner 2014).

Accession Number:P42956
Protein Name:PTS system, mannitol-specific IICBA component aka EIICBA-Mtl aka Mannitol-permease IIABC component aka Phosphotransferase enzyme II, CBA component aka EC 2.7.1.69 aka EII-Mtl
Length:478
Molecular Weight:50185.00
Species:Bacillus subtilis [1423]
Number of TMSs:9
Location1 / Topology2 / Orientation3: Cell membrane1 / Multi-pass membrane protein2
Substrate mannitol

Cross database links:

HEGENOM: HBG296680
RefSeq: NP_388280.2   
Entrez Gene ID: 938263   
Pfam: PF02378    PF02302   
KEGG: bsu:BSU03981   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0016301 F:kinase activity
GO:0008982 F:protein-N(PI)-phosphohistidine-sugar phosph...
GO:0005351 F:sugar:hydrogen symporter activity
GO:0009401 P:phosphoenolpyruvate-dependent sugar phospho...

References (4)

[1] “Determination of a 17,484 bp nucleotide sequence around the 39 degrees region of the Bacillus subtilis chromosome and similarity analysis of the products of putative ORFs.”  Akagawa E.et.al.   8574415
[2] “The 25 degrees-36 degrees region of the Bacillus subtilis chromosome: determination of the sequence of a 146 kb segment and identification of 113 genes.”  Yamane K.et.al.   8969502
[3] “The complete genome sequence of the Gram-positive bacterium Bacillus subtilis.”  Kunst F.et.al.   9384377
[4] “From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later.”  Barbe V.et.al.   19383706

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MQQQEQQQGG MKVKVQRFGS YLSGMIMPNI GAFIAWGIIT ALFIPAGWFP NEQLNTLVSP 
61:	MITYLLPLLI AYTGGKMIYD HRGGVVGATA AIGVIVGSDI PMFLGAMIMG PLGGYLIKQT 
121:	DKLFKDKVKQ GFEMLINNFT AGIVGAALTI LAFYAIGPVV LTLNKLLAAG VEVIVHANLL 
181:	PVASVFVEPA KVLFLNNAIN HGILSPIGIE QASQTGKSIL FLVEANPGPG LGILLAYMFF 
241:	GKGSSKSTAP GAAIIHFFGG IHEIYFPYIL MKPALILAAI AGGASGLLTF TIFNAGLVAA 
301:	ASPGSIIALM AMTPRGGYFG VLAGVLVAAA VSFIVSAVIL KSSKASEEDL AAATEKMQSM 
361:	KGKKSQAAAA LEAEQAKAEE ASELSPESVN KIIFACDAGM GSSAMGASIL RNKVKKAELD 
421:	ISVTNTAINN LPSDADIVIT HKDLTDRAKA KLPNATHISV DNFLNSPKYD ELIEKLKK