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3.A.1.12.2
Glycine betaine OpuAA/AB/AC porter (also transports dimethylsulfonioacetate and dimethylsulfoniopropionate).  The system has been reconstituted in nanodiscs and shows substrate-dependent ionic stringth-gated gating and energy coupling dependent on anionic lipids (Karasawa et al. 2013).

Accession Number:P46920
Protein Name:OPAA aka OpuAA
Length:418
Molecular Weight:46468.00
Species:Bacillus subtilis [1423]
Location1 / Topology2 / Orientation3: Cell inner membrane1 / Peripheral membrane protein2
Substrate Dimethylsulfoniopropionate, Dimethylsulfonioacetate, Glycine betaine

Cross database links:

HEGENOM: HBG758042
RefSeq: NP_388180.2   
Entrez Gene ID: 938362   
Pfam: PF00005    PF00571   
KEGG: bsu:BSU02980   

Gene Ontology

GO:0016020 C:membrane
GO:0015171 F:amino acid transmembrane transporter activity
GO:0005524 F:ATP binding
GO:0015418 F:quaternary-ammonium-compound-transporting A...
GO:0006865 P:amino acid transport

References (4)

[1] “OpuA, an osmotically regulated binding protein-dependent transport system for the osmoprotectant glycine betaine in Bacillus subtilis.”  Kempf B.et.al.   7622480
[2] “The 25 degrees-36 degrees region of the Bacillus subtilis chromosome: determination of the sequence of a 146 kb segment and identification of 113 genes.”  Yamane K.et.al.   8969502
[3] “The complete genome sequence of the Gram-positive bacterium Bacillus subtilis.”  Kunst F.et.al.   9384377
[4] “From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later.”  Barbe V.et.al.   19383706

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MSVDEKPIKI KVEKVSKIFG KQTKKAVQML ANGKTKKEIL KATGSTVGVN QADFEVYDGE 
61:	IFVIMGLSGS GKSTLVRMLN RLIEPTAGNI YIDGDMITNM SKDQLREVRR KKISMVFQKF 
121:	ALFPHRTILE NTEYGLELQG VDKQERQQKA LESLKLVGLE GFEHQYPDQL SGGMQQRVGL 
181:	ARALTNDPDI LLMDEAFSAL DPLIRKDMQD ELLDLHDNVG KTIIFITHDL DEALRIGDRI 
241:	VLMKDGNIVQ IGTPEEILMN PSNEYVEKFV EDVDLSKVLT AGHIMKRAET VRIDKGPRVA 
301:	LTLMKNLGIS SIYAVDKQKK LLGVIYASDA KKAAESDLSL QDILNTEFTT VPENTYLTEI 
361:	FDVVSDANIP IAVVDEKQRM KGIVVRGALI GALAGNNEYI NAEGTNEQTQ DPSAQEVK