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3.A.1.12.2
Glycine betaine OpuAA/AB/AC porter (also transports dimethylsulfonioacetate and dimethylsulfoniopropionate).  The system has been reconstituted in nanodiscs and shows substrate-dependent ionic stringth-gated gating and energy coupling dependent on anionic lipids (Karasawa et al. 2013).

Accession Number:P46921
Protein Name:OPAB aka OpuAB
Length:282
Molecular Weight:30247.00
Species:Bacillus subtilis [1423]
Number of TMSs:8
Location1 / Topology2 / Orientation3: Cell membrane1 / Multi-pass membrane protein2
Substrate Dimethylsulfoniopropionate, Dimethylsulfonioacetate, Glycine betaine

Cross database links:

HEGENOM: HBG592565
RefSeq: NP_388181.1   
Entrez Gene ID: 938360   
Pfam: PF00528   
KEGG: bsu:BSU02990   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0005215 F:transporter activity
GO:0006865 P:amino acid transport

References (3)

[1] “OpuA, an osmotically regulated binding protein-dependent transport system for the osmoprotectant glycine betaine in Bacillus subtilis.”  Kempf B.et.al.   7622480
[2] “The 25 degrees-36 degrees region of the Bacillus subtilis chromosome: determination of the sequence of a 146 kb segment and identification of 113 genes.”  Yamane K.et.al.   8969502
[3] “The complete genome sequence of the Gram-positive bacterium Bacillus subtilis.”  Kunst F.et.al.   9384377

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MDRLPRIPLA DIIDRFVDWI TMTFGGFFDG IANGLAAFVN GIVTGLGFIP SILLTIIFAA 
61:	LAWWISTRGI ALFTLIGFLL IDYLGYWDPM LQTLALVLTS VIISIVVGVP IGIWASQKET 
121:	VRRIVTPILD LMQTMPAFVY LLPAIFFFNI GVVPGVVASV IFAMPPTIRM TVLGIKQVPA 
181:	DLIEATEAFG STTAQRLFKV QLPLATKTIL AGINQSIMLA LSMVVIAAMV GAPGLGSEVY 
241:	SAVTQLKTGV GVEAGIAIVI VAITLDRITQ NIKVKKKSRG NA