TCDB is operated by the Saier Lab Bioinformatics Group
« See all members of the family


2.A.6.2.6
Multiple drug; N-(3-oxododecanoyl)- L-homoserine lactone autoinducer efflux pump, MexB (functions with MexA (an MFP, 8.A.1) and OprM (an OMF, 1.B.17; see 2.A.6.2.21). All three interact with each other. MexA promotes assembly and stability of the complex (Nehme and Poole, 2007)). Exports β-lactams, fluoroquinolones, tetracycline, macrolides, chloramphenicol, biocides, and a toxic indole compound, CBR-4830, that targets the MreB actin (Robertson et al., 2007). Confers tolerance to tea tree oil and its monoterpene components Terpinen-4-ol, 1,8-cineole and α-terpineol (Papadopoulos et al., 2008) as well as the antimicrobial peptide, colistin (Pamp et al., 2008) (Mao et al., 2002; Poole, 2008). The crystal structure has been solved at 3.0Å resolution (Sennhauser et al., 2009). The MexA-OprM complex has an elongated cylindrical appearance (Trépout et al., 2010).  Mutations affecting export of antibiotics with cytoplasmic targets have been identified (Ohene-Agyei et al. 2012). RND-type xenobiotic transporters recognize hydrophobic substrates such as organic solvents by their periplasmic domains and expel them to the external milieu (Li et al. 2006).

Accession Number:P52002
Protein Name:MexB aka PA0426
Length:1046
Molecular Weight:112791.00
Species:Pseudomonas aeruginosa [287]
Number of TMSs:12
Location1 / Topology2 / Orientation3: Cell inner membrane1 / Multi-pass membrane protein2
Substrate N-(3-oxododecanoyl)-L-homoserine lactone autoinducer

Cross database links:

HEGENOM: HBG293864
RefSeq: NP_249117.1   
Entrez Gene ID: 877852   
Pfam: PF00873   
Drugbank: Drugbank Link   
BioCyc: PAER208964:PA0426-MONOMER   
KEGG: pae:PA0426   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0005215 F:transporter activity
GO:0046677 P:response to antibiotic
GO:0006810 P:transport

References (8)

[1] “Cloning and sequence analysis of an EnvCD homologue in Pseudomonas aeruginosa: regulation by iron and possible involvement in the secretion of the siderophore pyoverdine.”  Poole K.et.al.   7968531
[2] “Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen.”  Stover C.K.et.al.   10984043
[3] “Molecular mechanisms of bacterial virulence elucidated using a Pseudomonas aeruginosa-Caenorhabditis elegans pathogenesis model.”  Mahajan-Miklos S.et.al.   9989496
[4] “Global analysis of the membrane subproteome of Pseudomonas aeruginosa using liquid chromatography-tandem mass spectrometry.”  Blonder J.et.al.   15253424
[5] “Multiple antibiotic resistance in Pseudomonas aeruginosa: evidence for involvement of an efflux operon.”  Poole K.et.al.   8226684
[6] “Role of mexA-mexB-oprM in antibiotic efflux in Pseudomonas aeruginosa.”  Li X.-Z.et.al.   8540696
[7] “Role of the multidrug efflux systems of Pseudomonas aeruginosa in organic solvent tolerance.”  Li X.-Z.et.al.   9603892
[8] “Membrane topology of the xenobiotic-exporting subunit, MexB, of the MexA,B-OprM extrusion pump in Pseudomonas aeruginosa.”  Guan L.et.al.   10187844
Structure:
2V50   3W9I   3W9J     

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
Window Size: Angle:  
Window Size: Angle:  
FASTA formatted sequence
1:	MSKFFIDRPI FAWVIALVIM LAGGLSILSL PVNQYPAIAP PAIAVQVSYP GASAETVQDT 
61:	VVQVIEQQMN GIDNLRYISS ESNSDGSMTI TVTFEQGTDP DIAQVQVQNK LQLATPLLPQ 
121:	EVQRQGIRVT KAVKNFLMVV GVVSTDGSMT KEDLSNYIVS NIQDPLSRTK GVGDFQVFGS 
181:	QYSMRIWLDP AKLNSYQLTP GDVSSAIQAQ NVQISSGQLG GLPAVKGQQL NATIIGKTRL 
241:	QTAEQFENIL LKVNPDGSQV RLKDVADVGL GGQDYSINAQ FNGSPASGIA IKLATGANAL 
301:	DTAKAIRQTI ANLEPFMPQG MKVVYPYDTT PVVSASIHEV VKTLGEAILL VFLVMYLFLQ 
361:	NFRATLIPTI AVPVVLLGTF GVLAAFGFSI NTLTMFGMVL AIGLLVDDAI VVVENVERVM 
421:	AEEGLSPREA ARKSMGQIQG ALVGIAMVLS AVFLPMAFFG GSTGVIYRQF SITIVSAMAL 
481:	SVIVALILTP ALCATMLKPI EKGDHGEHKG GFFGWFNRMF LSTTHGYERG VASILKHRAP 
541:	YLLIYVVIVA GMIWMFTRIP TAFLPDEDQG VLFAQVQTPP GSSAERTQVV VDSMREYLLE 
601:	KESSSVSSVF TVTGFNFAGR GQSSGMAFIM LKPWEERPGG ENSVFELAKR AQMHFFSFKD 
661:	AMVFAFAPPS VLELGNATGF DLFLQDQAGV GHEVLLQARN KFLMLAAQNP ALQRVRPNGM 
721:	SDEPQYKLEI DDEKASALGV SLADINSTVS IAWGSSYVND FIDRGRVKRV YLQGRPDARM 
781:	NPDDLSKWYV RNDKGEMVPF NAFATGKWEY GSPKLERYNG VPAMEILGEP APGLSSGDAM 
841:	AAVEEIVKQL PKGVGYSWTG LSYEERLSGS QAPALYALSL LVVFLCLAAL YESWSIPFSV 
901:	MLVVPLGVIG ALLATSMRGL SNDVFFQVGL LTTIGLSAKN AILIVEFAKE LHEQGKGIVE 
961:	AAIEACRMRL RPIVMTSLAF ILGVVPLAIS TGAGSGSQHA IGTGVIGGMV TATVLAIFWV 
1021:	PLFYVAVSTL FKDEASKQQA SVEKGQ