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2.A.6.2.6
Multiple drug; N-(3-oxododecanoyl)- L-homoserine lactone autoinducer efflux pump, MexB (functions with MexA (an MFP, 8.A.1) and OprM (an OMF, 1.B.17; see 2.A.6.2.21). All three interact with each other. MexA promotes assembly and stability of the complex (Nehme and Poole, 2007)). Exports β-lactams, fluoroquinolones, tetracycline, macrolides, chloramphenicol, biocides, and a toxic indole compound, CBR-4830, that targets the MreB actin (Robertson et al., 2007). Confers tolerance to tea tree oil and its monoterpene components Terpinen-4-ol, 1,8-cineole and α-terpineol (Papadopoulos et al., 2008) as well as the antimicrobial peptide, colistin (Pamp et al., 2008) (Mao et al., 2002; Poole, 2008). The crystal structure has been solved at 3.0Å resolution (Sennhauser et al., 2009). The MexA-OprM complex has an elongated cylindrical appearance (Trépout et al., 2010).  Mutations affecting export of antibiotics with cytoplasmic targets have been identified (Ohene-Agyei et al. 2012). RND-type xenobiotic transporters recognize hydrophobic substrates such as organic solvents by their periplasmic domains and expel them to the external milieu (Li et al. 2006).

Accession Number:P52477
Protein Name:Multidrug resistance protein MexA
Length:383
Molecular Weight:40970.00
Species:Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228) [208964]
Location1 / Topology2 / Orientation3: Cell inner membrane1 / Lipid-anchor2
Substrate N-(3-oxododecanoyl)-L-homoserine lactone autoinducer

Cross database links:

Drugbank: Drugbank Link   
Structure:
1T5E   1VF7   2V4D   4DK1     

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MQRTPAMRVL VPALLVAISA LSGCGKSEAP PPAQTPEVGI VTLEAQTVTL NTELPGRTNA 
61:	FRIAEVRPQV NGIILKRLFK EGSDVKAGQQ LYQIDPATYE ADYQSAQANL ASTQEQAQRY 
121:	KLLVADQAVS KQQYADANAA YLQSKAAVEQ ARINLRYTKV LSPISGRIGR SAVTEGALVT 
181:	NGQANAMATV QQLDPIYVDV TQPSTALLRL RRELASGQLE RAGDNAAKVS LKLEDGSQYP 
241:	LEGRLEFSEV SVDEGTGSVT IRAVFPNPNN ELLPGMFVHA QLQEGVKQKA ILAPQQGVTR 
301:	DLKGQATALV VNAQNKVELR VIKADRVIGD KWLVTEGLNA GDKIITEGLQ FVQPGVEVKT 
361:	VPAKNVASAQ KADAAPAKTD SKG