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4.A.7.1.1
The L-ascorbate transporting and phosphorylating group translocator, SgaTBA or UlaCBA (SgaT = UlaA = YjfS; SgaB = UlaB = YjfT; SgaA = UlaC = PtxA = YjfU) (Hvorup et al., 2003; Zhang et al., 2003). Two conformations of the 3-d structure have been determined at 1.65 and 2.35 Å resolution, respectively (Luo et al., 2015). UlaA (SgaT) forms a homodimer with a novel fold. Each UlaA protomer consists of 11 TMSs arranged into a 'V-motif' domain and a 'core' domain. The V motifs form the interface between the two protomers, and the core-domain residues coordinate vitamin C. Alternating access of the substrate to the two sides of the cell membrane may be achieved through rigid-body rotation of the core relative to the V motif (Luo et al., 2015; Zhang et al., 2003). This structure does not resemble the ChbC structure (TC# 4.A.3.2.8).

Accession Number:P69820
Protein Name:SgaA aka B4195
Length:154
Molecular Weight:17238.00
Species:Escherichia coli [83333]
Location1 / Topology2 / Orientation3: Cytoplasm1
Substrate Ascorbate

Cross database links:

Genevestigator: P69820
EchoBASE: EB2388
EcoGene: EG12495
eggNOG: COG1762
HEGENOM: HBG697201
RefSeq: AP_004695.1    NP_418616.1   
Entrez Gene ID: 948715   
Pfam: PF00359   
BioCyc: EcoCyc:YJFU-MONOMER    ECOL168927:B4195-MONOMER   
KEGG: ecj:JW4153    eco:b4195   

Gene Ontology

GO:0005737 C:cytoplasm
GO:0016301 F:kinase activity
GO:0008982 F:protein-N(PI)-phosphohistidine-sugar phosph...
GO:0005351 F:sugar:hydrogen symporter activity
GO:0009401 P:phosphoenolpyruvate-dependent sugar phospho...

References (6)

[1] “Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes.”  Burland V.D.et.al.   7610040
[2] “The complete genome sequence of Escherichia coli K-12.”  Blattner F.R.et.al.   9278503
[3] “Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.”  Hayashi K.et.al.   16738553
[4] “The gene yjfQ encodes the repressor of the yjfR-X regulon (ula), which is involved in L-ascorbate metabolism in Escherichia coli.”  Campos E.et.al.   12374842
[5] “The ascorbate transporter of Escherichia coli.”  Zhang Z.et.al.   12644495
[6] “Regulation of expression of the divergent ulaG and ulaABCDEF operons involved in L-ascorbate dissimilation in Escherichia coli.”  Campos E.et.al.   14996803

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MKLRDSLAEN KSIRLQAEAE TWQEAVKIGV DLLVAADVVE PRYYQAILDG VEQFGPYFVI 
61:	APGLAMPHGR PEEGVKKTGF SLVTLKKPLE FNHDDNDPVD ILITMAAVDA NTHQEVGIMQ 
121:	IVNLFEDEEN FDRLRACRTE QEVLDLIDRT NAAA