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3.A.1.125.1
Lipoprotein translocation system (translocates lipoproteins from the inner membrane to periplasmic chaperone, LolA, which transfers the lipoproteins to an outer membrane receptor, LolB, which anchors the lipoprotein to the outer membrane of the Gram-negative bacterial cell envelope) (see 1.B.46; Narita et al., 2003; Ito et al., 2006; Watanabe et al., 2007). The structure of ligand-bound LolCDE has been solved (Ito et al., 2006). LolC and LolE each have 4 TMSs (1+3). Unlike most ATP binding cassette transporters mediating the transmembrane flux of substrates, the LolCDE complex catalyzes the extrusion of lipoproteins anchored to the outer leaflet of the inner membrane. The LolCDE complex is unusual in that it can be purified as a liganded form, which is an intermediate of the lipoprotein release reaction (Taniguchi and Tokuda, 2008). LolCDE has been reconstituted from separated subunits (Kanamaru et al., 2007).  LolE binds the outer membrane lipoprotein, PAL (Mizutani et al. 2013).

Accession Number:P75957
Protein Name:LolD aka B1117
Length:233
Molecular Weight:25438.00
Species:Escherichia coli [83333]
Number of TMSs:1
Location1 / Topology2 / Orientation3: Cell inner membrane1 / Peripheral membrane protein2
Substrate Lipoproteins

Cross database links:

Genevestigator: P75957
EchoBASE: EB3214
EcoGene: EG13440
eggNOG: COG1136
HEGENOM: HBG758042
DIP: DIP-6893N
RefSeq: AP_001743.1    NP_415635.4   
Entrez Gene ID: 945670   
Pfam: PF00005   
BioCyc: EcoCyc:YCFV-MONOMER    ECOL168927:B1117-MONOMER   
KEGG: ecj:JW5162    eco:b1117   

Gene Ontology

GO:0005886 C:plasma membrane
GO:0005524 F:ATP binding
GO:0016887 F:ATPase activity
GO:0042954 F:lipoprotein transporter activity
GO:0042953 P:lipoprotein transport

References (5)

[1] “A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map.”  Oshima T.et.al.   8905232
[2] “The complete genome sequence of Escherichia coli K-12.”  Blattner F.R.et.al.   9278503
[3] “Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.”  Hayashi K.et.al.   16738553
[4] “A new ABC transporter mediating the detachment of lipid-modified proteins from membranes.”  Yakushi T.et.al.   10783239
[5] “Disruption of lolCDE, encoding an ATP-binding cassette transporter, is lethal for Escherichia coli and prevents release of lipoproteins from the inner membrane.”  Narita S.et.al.   11844772

External Searches:

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MNKILLQCDN LCKRYQEGSV QTDVLHNVSF SVGEGEMMAI VGSSGSGKST LLHLLGGLDT 
61:	PTSGDVIFNG QPMSKLSSAA KAELRNQKLG FIYQFHHLLP DFTALENVAM PLLIGKKKPA 
121:	EINSRALEML KAVGLDHRAN HRPSELSGGE RQRVAIARAL VNNPRLVLAD EPTGNLDARN 
181:	ADSIFQLLGE LNRLQGTAFL VVTHDLQLAK RMSRQLEMRD GRLTAELSLM GAE