TCDB is operated by the Saier Lab Bioinformatics Group
« See all members of the family


3.A.1.15.8
Manganese (Mn2+), zinc (Zn2+) and possibly iron (Fe2+) uptake porter, TroABCD (Hazlett et al., 2003).  Transcription of the operon is controlled by the Mn2+-activated (not Zn2+- or Fe2+-activated) repressor, TroR (153 aas, acc# F7IW50;) TroR contains a metal-binding domain homologous to the YtgC-R protein (3.A.1.15.12) which has the membrane domain of this ABC transporter (N-terminus) fused to the repressor domain (C-terminus) (Liu et al. 2013).  TroA (Tromp1), the periplasmic metal binding protein, was originally reported to be an outer membrane porin (Zhang et al. 1999), but this proved to be incorrect.

Accession Number:P96116
Protein Name:TroA
Length:308
Molecular Weight:33570.00
Species:Treponema pallidum [160]
Number of TMSs:1
Location1 / Topology2 / Orientation3: Periplasm1
Substrate Zn2+, Mn2+, Fe2+

Cross database links:

HEGENOM: HBG731008
RefSeq: NP_218602.1   
Entrez Gene ID: 2611122   
Pfam: PF01297   
BioCyc: TPAL243276:TP_0163-MONOMER   
KEGG: tpa:TP0163   

Gene Ontology

GO:0042597 C:periplasmic space
GO:0046872 F:metal ion binding
GO:0007155 P:cell adhesion
GO:0006829 P:zinc ion transport

References (5)

[1] “Porin activity and sequence analysis of a 31-kilodalton Treponema pallidum subsp. pallidum rare outer membrane protein (Tromp1).”  Blanco D.R.et.al.   7768866
[2] “Identification and transcriptional analysis of a Treponema pallidum operon encoding a putative ABC transport system, an iron-activated repressor protein homolog, and a glycolytic pathway enzyme homolog.”  Hardham J.M.et.al.   9332349
[3] “Complete genome sequence of Treponema pallidum, the syphilis spirochete.”  Fraser C.M.et.al.   9665876
[4] “Physicochemical evidence that Treponema pallidum TroA is a zinc-containing metalloprotein that lacks porin-like structure.”  Deka R.K.et.al.   10400603
[5] “Treponema pallidum TroA is a periplasmic zinc-binding protein with a helical backbone.”  Lee Y.-H.et.al.   10404217
Structure:
1K0F   1TOA     

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
Window Size: Angle:  
Window Size: Angle:  
FASTA formatted sequence
1:	MIRERICACV LALGMLTGFT HAFGSKDAAA DGKPLVVTTI GMIADAVKNI AQGDVHLKGL 
61:	MGPGVDPHLY TATAGDVEWL GNADLILYNG LHLETKMGEV FSKLRGSRLV VAVSETIPVS 
121:	QRLSLEEAEF DPHVWFDVKL WSYSVKAVYE SLCKLLPGKT REFTQRYQAY QQQLDKLDAY 
181:	VRRKAQSLPA ERRVLVTAHD AFGYFSRAYG FEVKGLQGVS TASEASAHDM QELAAFIAQR 
241:	KLPAIFIESS IPHKNVEALR DAVQARGHVV QIGGELFSDA MGDAGTSEGT YVGMVTHNID 
301:	TIVAALAR