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8.A.30.1.1
Nedd4-2 (1004 aas, 0 TMSs: Ubiquitin protein ligase, E3)/Nedd4 interacting protein-2 (311 aas; 3 TMSs: ww domain binding protein 5 (N4WBP5A)) (Zhou, et al., 2007)

Accession Number:Q8CFI0
Protein Name:E3 ubiquitin-protein ligase NEDD4-like protein aka Nedd4-2
Length:1004
Molecular Weight:115419.00
Species:Mus musculus (Mouse) [10090]
Location1 / Topology2 / Orientation3: Cytoplasm1
Substrate NONE

Cross database links:

Genevestigator: Q8CFI0
HEGENOM: HBG607874
Pfam: PF00168    PF00632    PF00397   

Gene Ontology

GO:0005737 C:cytoplasm
GO:0016881 F:acid-amino acid ligase activity
GO:0005515 F:protein binding
GO:0019871 F:sodium channel inhibitor activity
GO:0010766 P:negative regulation of sodium ion transport
GO:0006464 P:protein modification process

References (12)

[1] “A novel mouse Nedd4 protein suppresses the activity of the epithelial Na+ channel.”  Kamynina E.et.al.   11149908
[2] “The role of individual Nedd4-2 (KIAA0439) WW domains in binding and regulating epithelial sodium channels.”  Fotia A.B.et.al.   12424229
[3] “The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).”  The MGC Project Teamet.al.   15489334
[4] “The transcriptional landscape of the mammalian genome.”  Carninci P.et.al.   16141072
[5] “Phosphorylation of Nedd4-2 by Sgk1 regulates epithelial Na(+) channel cell surface expression.”  Debonneville C.et.al.   11742982
[6] “The Nedd4-like protein KIAA0439 is a potential regulator of the epithelial sodium channel.”  Harvey K.F.et.al.   11244092
[7] “Regulation of the epithelial sodium channel by N4WBP5A, a novel Nedd4/Nedd4-2-interacting protein.”  Konstas A.-A.et.al.   12050153
[8] “Regulation of neuronal voltage-gated sodium channels by the ubiquitin-protein ligases Nedd4 and Nedd4-2.”  Fotia A.B.et.al.   15123669
[9] “Participation of the ubiquitin-conjugating enzyme UBE2E3 in Nedd4-2-dependent regulation of the epithelial Na+ channel.”  Debonneville C.et.al.   14993279
[10] “Aldosterone-induced serum and glucocorticoid-induced kinase 1 expression is accompanied by nedd4-2 phosphorylation and increased na+ transport in cortical collecting duct cells.”  Flores S.Y.et.al.   15958725
[11] “Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations.”  Munton R.P.et.al.   17114649
[12] “Large-scale phosphorylation analysis of mouse liver.”  Villen J.et.al.   17242355
Structure:
1WR3   1WR4   1WR7     

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MSLCEAPVHV GDKELKYFQI PQMLSQLSLL ASHHSRGLEF SGGQGESRIL RVKVVSGIDL 
61:	AKKDIFGASD PYVKLSLYVA DENRELALVQ TKTIKKTLNP KWNEEFYFRV NPSNHRLLFE 
121:	VFDENRLTRD DFLGQVDVPL SHLPTEDPTM ERPYTFKDFL LRPRSHKSRV KGFLRLKMAY 
181:	MPKNGGQDEE NSEQRDDMEH GWEVVDSNDS ASQHQEELPP PPLPPGWEEK VDNLGRTYYV 
241:	NHNNRSTQWH RPSLMDVSSE SDNNIRQINQ EAAHRRFRSR RHISEDLEPE ASEGGGEGPE 
301:	PWETISEEMN MAGDSLSLAL PPPPASPVSR TSPQELSEEV SRRLQITPDS NGEQFSSLIQ 
361:	REPSSRLRSC SVTDTVAEQA HLPPPSTPTR RARSSTVTGG EEPTPSVAYV HTTPGLPSGW 
421:	EERKDAKGRT YYVNHNNRTT TWTRPIMQLA EDGASGSATN SNNHLVEPQI RRPRSLSSPT 
481:	VTLSAPLEGA KDSPIRRAVK DTLSNPQSPQ PSPYNSPKPQ HKVTQSFLPP GWEMRIAPNG 
541:	RPFFIDHNTK TTTWEDPRLK FPVHMRSKAS LNPNDLGPLP PGWEERIHLD GRTFYIDHNS 
601:	KITQWEDPRL QNPAITGPAV PYSREFKQKY DYFRKKLKKP ADIPNRFEMK LHRNNIFEES 
661:	YRRIMSVKRP DVLKARLWIE FESEKGLDYG GVAREWFFLL SKEMFNPYYG LFEYSATDNY 
721:	TLQINPNSGL CNEDHLSYFT FIGRVAGLAV FHGKLLDGFF IRPFYKMMLG KQITLNDMES 
781:	VDSEYYNSLK WILENDPTEL DLMFCIDEEN FGQTYQVDLK PNGSEIMVTN ENKREYIDLV 
841:	IQWRFVNRVQ KQMNAFLEGF TELLPIDLIK IFDENELELL MCGLGDVDVN DWRQHSIYKN 
901:	GYCPNHPVIQ WFWKAVLLMD AEKRIRLLQF VTGTSRVPMN GFAELYGSNG PQLFTIEQWG 
961:	SPEKLPRAHT CFNRLDLPPY ETFEDLREKL LMAVENAQGF EGVD