TCDB is operated by the Saier Lab Bioinformatics Group
« See all members of the family


5.B.5.1.1
The multihaem c-type cytochrome quinol:Fe3+ /Mn3 /4+ oxidoreductase, Cym/Mtr (Shi et al., 2007).  MtrABC is composed of two decahaem cytochromes (MtrA & B) brought together inside a transmembrane β-porin (MtrC) to transport electrons across the outer membrane to mineral based electron acceptors (White et al. 2012).  Conduction through MtrCAB directly to Fe(III) oxides occurs both in vitro in liposomes and in vivo, allowing anaerobic, solid-phase iron respiration (White et al. 2013). MtrC interacts with the surface of MtrAB, extending ∼70 Å from the membrane surface and allowing the terminal hemes to interact with both MtrAB and an extracellular acceptor. MtrA fully extends through the length of MtrB, with ∼30 Å being exposed into the periplasm. MtrCAB can reduce Fe(III) citrate with STC as an electron donor, disclosing a direct interaction between MtrCAB and STC (Edwards et al. 2018). MtrC, but not UndA (a paralog of MtrC of 843 aas; F8UWD6), appears to be the primary reductase of flavins to ensure fast indirect extracellular electron transfer (EET), which plays a crucial role in microbial fuel cell (MFC) electricity generation in Shewanella putrefaciens CN32 (Wu et al. 2018). The dimensions of MtrAB are approximately 105 x 60 x 35 Å and approximately 170 x 60 x 45 Å for MtrCAB. Their shapes suggest that MtrC interacts with the surface of MtrAB, extending approximately 70 Å from the membrane surface and allowing the terminal hemes to interact with both MtrAB and an extracellular acceptor. MtrA fully extends through the length of MtrB, with approximately 30 Å being exposed into the periplasm (Edwards et al. 2018).

Accession Number:Q8EG35
Protein Name:MtrA
Length:333
Molecular Weight:36059.00
Species:Shewanella oneidensis [70863]
Number of TMSs:1
Location1 / Topology2 / Orientation3: Cell inner membrane1 / Multi-pass membrane protein2
Substrate Electrons

Cross database links:

HEGENOM: HBG496765
RefSeq: NP_717386.1   
Entrez Gene ID: 1169551   
BioCyc: SONE211586:SO_1777-MONOMER   
KEGG: son:SO_1777   

References (1)

[1] “Genome sequence of the dissimilatory metal ion-reducing bacterium Shewanella oneidensis.”  Heidelberg J.F.et.al.   12368813

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
Window Size: Angle:  
Window Size: Angle:  
FASTA formatted sequence
1:	MKNCLKMKNL LPALTITMAM SAVMALVVTP NAYASKWDEK MTPEQVEATL DKKFAEGNYS 
61:	PKGADSCLMC HKKSEKVMDL FKGVHGAIDS SKSPMAGLQC EACHGPLGQH NKGGNEPMIT 
121:	FGKQSTLSAD KQNSVCMSCH QDDKRMSWNG GHHDNADVAC ASCHQVHVAK DPVLSKNTEM 
181:	EVCTSCHTKQ KADMNKRSSH PLKWAQMTCS DCHNPHGSMT DSDLNKPSVN DTCYSCHAEK 
241:	RGPKLWEHAP VTENCVTCHN PHGSVNDGML KTRAPQLCQQ CHASDGHASN AYLGNTGLGS 
301:	NVGDNAFTGG RSCLNCHSQV HGSNHPSGKL LQR