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3.A.7.17.1
The EsxA/B protein secretion pathway system (EPSP) (Anderson et al. 2011; Burts et al. 2005; Chen et al. 2012; Sundaramoorthy et al. 2008).  EssC is an integral component of the bacterial """"Type VII"""" secretion system (T7SS). The protein is predicted to consist of an intracellular repeat of forkhead-associated (FHA) domains at the N-terminus, two transmembrane helices and three P-loop containing ATPase-type domains, D1-3, forming the C-terminal intracellular segment. Zoltner et al. 2016 presented crystal structures of the N-terminal FHA domains (EssC-N) and a C-terminal fragment EssC-C from Geobacillus thermodenitrificans, encompassing two of the ATPase-type modules, D2 and D3. Module D2 binds ATP with high affinity whereas D3 does not. The EssC-N and EssC-C constructs are monomeric in solution but the full-length recombinant protein, with molecular weight about 169 kDa, forms a multimer of approximately 1 MDa. The observation of protomer contacts in the crystal structure of EssC-C together with similarity to the DNA translocase FtsK, suggests a model for a hexameric EssC assembly. Such an observation potentially identifies the key component of pore formation required for secretion. The extreme C-terminal ATPase domain appears essential for EssC activity as a key part of the T7SS whilst D2 and FHA domains are required for the production of a stable and functional protein (Zoltner et al. 2016). TheTMSs of EssA and EssB interact, regulating secretion (Ahmed et al. 2018). This system resembles the ESX systems of Mycobacterium tuberculosis (TC# 3.A.24).  

Accession Number:Q932J9
Protein Name:EssC
Length:1479
Molecular Weight:170932.00
Species:Staphylococcus aureus (strain Mu50 / ATCC 700699) [158878]
Number of TMSs:2
Location1 / Topology2 / Orientation3: Cell membrane1 / Multi-pass membrane protein2
Substrate EsxA, EsxB

Cross database links:

eggNOG: COG1674
HEGENOM: HBG417611
RefSeq: NP_370811.2   
Entrez Gene ID: 1120245   
Pfam: PF01580    PF12538   
BioCyc: SAUR158878:SAV0287-MONOMER   
KEGG: sav:SAV0287   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0005524 F:ATP binding
GO:0003677 F:DNA binding
GO:0007049 P:cell cycle
GO:0051301 P:cell division
GO:0007059 P:chromosome segregation
GO:0009405 P:pathogenesis

References (1)

[1] “Whole genome sequencing of meticillin-resistant Staphylococcus aureus.”  Kuroda M.et.al.   11418146

External Searches:

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  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MHKLIIKYNK QLKMLNLRDG KTYTISEDER ADITLKSLGE VIHLEQNNQG TWQANHTSIN 
61:	KVLVRKGDLD DITLQLYTEA DYASFAYPSI QDTMTIGPNA YDDMVIQSLM NAIIIKDFQS 
121:	IQESQYVRIV HDKNTDVYIN YELQEQLTNK AYIGDHIYVE GIWLEVQADG LNVLSQNTVA 
181:	SSLIRLTQEM PHAQADDYNT YHRSPRIIHR EPTDDIKIER PPQPIQKNNT VIWRSIIPPL 
241:	VMIALTVVIF LVRPIGIYIL MMIGMSTVTI VFGITTYFSE KKKYNKDVEK REKDYKAYLD 
301:	NKSKEINKAI KAQRFSLNYH YPTVAEIKDI VETKAPRIYE KTSHHHDFLH YKLGIANVEK 
361:	SFKLDYQEEE FNQRRDELFD DAKELYEFYT DVEQAPLIND LNHGPIAYIG ARHLILEELE 
421:	KMLIQLSTFH SYHDLEFLFV TREDEVETLK WARWLPHMTL RGQNIRGFVY NQRTRDQILT 
481:	SIYSMIKERI QAVRERSRSN EQIIFTPQLV FVITDMSLII DHVILEYVNQ DLSEYGISLI 
541:	FVEDVIESLP EHVDTIIDIK SRTEGELITK EKELVQLKFT PENIDNVDKE YIARRLANLI 
601:	HVEHLKNAIP DSITFLEMYN VKEVDQLDVV NRWRQNETYK TMAVPLGVRG KDDILSLNLH 
661:	EKAHGPHGLV AGTTGSGKSE IIQSYILSLA INFHPHEVAF LLIDYKGGGM ANLFKDLVHL 
721:	VGTITNLDGD EAMRALTSIK AELRKRQRLF GEHDVNHINQ YHKLFKEGVA TEPMPHLFII 
781:	SDEFAELKSE QPDFMKELVS TARIGRSLGI HLILATQKPS GVVDDQIWSN SKFKLALKVQ 
841:	DRQDSNEILK TPDAADITLP GRAYLQVGNN EIYELFQSAW SGATYDIEGD KLEVEDKTIY 
901:	MINDYGQLQA INKDLSGLED EETKENQTEL EAVIDHIESI TTRLEIEEVK RPWLPPLPEN 
961:	VYQEDLVETD FRKLWSDDAK EVELTLGLKD VPEEQYQGPM VLQLKKAGHI ALIGSPGYGR 
1021:	TTFLHNIIFD VARHHRPDQA HMYLFDFGTN GLMPVTDIPH VADYFTVDQE DKIAKAIRIF 
1081:	NDEIDRRKKI LSQYRVTSIS EYRKLTGETI PYVFILIDNF DAVKDSPFQE VFENMMIKMT 
1141:	REGLALDMQV TLTASRANAM KTPMYINMKT RIAMFLYDKS EVSNVVGQQK FAVKDVVGRA 
1201:	LLSSDDNVSF HIGQPFKHDE TKSYNDQIND EVSAMTEFYK GETPNDIPMM PDEIKYEDYR 
1261:	ESLSLPDIVA NGALPIGLDY EGVTLQKIKL TEPAMISSEN PREIAHIAEI MMKEIDILNE 
1321:	KYAICIADSS GEFKAYRHQV ANFAEEREDI KAIHQLMIED LKQREMDGPF EKDSLYIIND 
1381:	FKTYIDCTYI PEDDVKKLIT KGPELGLNIL FVGIHKELID AYDKQIDVAR KMINQFSIGI 
1441:	RISDQQFFKF RFIQREPVIK ENEAYMVANQ AYQKIRWFK