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3.A.7.17.1
The EsxA/B protein secretion pathway system (EPSP) (Anderson et al. 2011; Burts et al. 2005; Chen et al. 2012; Sundaramoorthy et al. 2008).  EssC is an integral component of the bacterial """"Type VII"""" secretion system (T7SS). The protein is predicted to consist of an intracellular repeat of forkhead-associated (FHA) domains at the N-terminus, two transmembrane helices and three P-loop containing ATPase-type domains, D1-3, forming the C-terminal intracellular segment. Zoltner et al. 2016 presented crystal structures of the N-terminal FHA domains (EssC-N) and a C-terminal fragment EssC-C from Geobacillus thermodenitrificans, encompassing two of the ATPase-type modules, D2 and D3. Module D2 binds ATP with high affinity whereas D3 does not. The EssC-N and EssC-C constructs are monomeric in solution but the full-length recombinant protein, with molecular weight about 169 kDa, forms a multimer of approximately 1 MDa. The observation of protomer contacts in the crystal structure of EssC-C together with similarity to the DNA translocase FtsK, suggests a model for a hexameric EssC assembly. Such an observation potentially identifies the key component of pore formation required for secretion. The extreme C-terminal ATPase domain appears essential for EssC activity as a key part of the T7SS whilst D2 and FHA domains are required for the production of a stable and functional protein (Zoltner et al. 2016). TheTMSs of EssA and EssB interact, regulating secretion (Ahmed et al. 2018). This system resembles the ESX systems of Mycobacterium tuberculosis (TC# 3.A.24).  

Accession Number:Q99WU3
Protein Name:EsaA
Length:1009
Molecular Weight:114782.00
Species:Staphylococcus aureus (strain Mu50 / ATCC 700699) [158878]
Number of TMSs:6
Location1 / Topology2 / Orientation3: Cell membrane1 / Multi-pass membrane protein2
Substrate EsxA, EsxB

Cross database links:

eggNOG: COG1511
HEGENOM: HBG216040
RefSeq: NP_370807.1   
Entrez Gene ID: 1120241   
BioCyc: SAUR158878:SAV0283-MONOMER   
KEGG: sav:SAV0283   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0009405 P:pathogenesis

References (1)

[1] “Whole genome sequencing of meticillin-resistant Staphylococcus aureus.”  Kuroda M.et.al.   11418146

External Searches:

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  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MKKKNWIYAL IVTLIIIIAI VSMIFFVQTK YGDQSEKGSQ SVSNKNNKIH IAIVNEDQPT 
61:	TYNGKKVELG QAFIKRLANE KNYKFETVTR NVAESGLKNG GYQVMIVIPE NFSKLAMQLD 
121:	AKTPSKISLQ YKTAVGQKEE VAKNTEKVVS NVLNDFNKNL VEIYLTSIID NLHNAQKNVG 
181:	AIMTREHGVN SKFSNYLLNP INDFPELFTD TLVNSISANK DITKWFQTYN KSLLSANSDT 
241:	FRVNTDYNVS TLIEKQNSLF DEHNTAMDKM LQDYKSQKDS VELDNYINAL KQMDSQIDQQ 
301:	SSMQDTGKEE YKQTVKENLD KLREIIQSQE SPFSKGMIED YRKQLTESLQ DELANNKDLQ 
361:	DALNSIKMNN AQFAENLEKQ LHDDIVKEPD TDTTFIYNMS KQDFIAAGLN EGEANKYEAI 
421:	VKEAKRYKNE YNLKKPLAEH INLTDYDNQV AQDTSSLIND GVKVQRTETI KSNDINQLTV 
481:	ATDPHFNFEG DIKINGKKYD IKDQSVQLDT SNKEYKVEVN GVAKLKKDAE KDFLKDKTMH 
541:	LQLLFGQANR QDEPNDKKAT SVVDVTLNHN LDGRLSKDAL SQQLSALSRF DAHYKMYTDT 
601:	KGREDKPFDN KRLIDMMVDQ VINDMESFKD DKVAVLHQID SMEENSDKLI DDILNNKKNT 
661:	TKNKEDISKL IDQLENVKKT FAEEPQEPKI DKGKNDEFNT MSSNLDKEIS RISEKSTQLL 
721:	SDTQESKTIA DSVSGQLNQL DNNVNKLHAT GRALGVRAND LNRQMAKNDK DNELFAKEFK 
781:	KVLQNSKDGD RQNQALKAFM SNPVQKKNLE NVLANNGNTE VISPTLFVLL MYLLSMITAY 
841:	IFYSYERAKG QMNFIKDDYS SKNHLWNNVI TSGVIGTTGL VEGLIVGLIA MNKFHVLAGY 
901:	RAKFILMVIL TMMVFVLINT YLLRQVKSIG MFLMIAALGL YFVAMNNLKA AGQGVTNKIS 
961:	PLSYIDNMFF NYLNAEHPIG LALVILTVLV IIGFVLNMFI KHFKKERLI