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2.A.6.2.10
Solvent efflux pump, TtgDEF (extrudes only toluene and styrene) (Teran et al., 2007).

Accession Number:Q9KWV4
Protein Name:TtgE
Length:1048
Molecular Weight:113676.00
Species:Pseudomonas putida [303]
Number of TMSs:12
Location1 / Topology2 / Orientation3: Cell inner membrane1 / Multi-pass membrane protein2
Substrate Toluene, Styrene

Cross database links:

Pfam: PF00873   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0005215 F:transporter activity
GO:0006810 P:transport

References (2)

[1] “A set of genes encoding a second toluene efflux system in Pseudomonas putida DOT-T1 is linked to the tod genes for toluene metabolism.”  Mosqueda G.et.al.   10648517
[2] “Global and cognate regulators control the expression of the organic solvent efflux pumps TtgABC and TtgDEF of Pseudomonas putida.”  Duque E.et.al.   11251828

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MSRFFIDRPI FAWVLAIIAM LAGALSLTKM PISQYPNIAA PAVSIQVVYP GASAKTVQDT 
61:	VVQVIEQQLN GLDGFRYMAA ESASDGSMNI IVTFEQGTNP DIAQVQVQNK LQLATPRLPE 
121:	EVQRQGLRVV KYQMNFFMVV GLVDKTGKMT NFDLGNLIAS QLQDPISRIN GVGDFLLFGS 
181:	PYAMRIWLDP GKLNSYQLTP GDVAQAIREQ NVQVSSGQLG GLPTRSGVQL NATVVGKTRM 
241:	TTPAEFEEIL VKVKADGSQV RVKDLDRVVL ASENFAISAK YRGQDSAGLG LGLRLASGGN 
301:	LLETVKAVKA ELEKQKAYLP EGVEVIYPYD TSPVVEASID SVVHTILEAV VLVFLVMFLF 
361:	LQSLRATIIP TLAVPVVLLA AFALLPYFGI SINVLTMYAM VLAIGLLVDD AIVVVENVER 
421:	LMHDEGLSPL EATRKSMGQI SGALVGIGMV LSAVFVPMAF FGGSAGIIYK QFAVTIVICM 
481:	SLSVLVALIF TPALCATILK APENDAHHEK KGFFGWFNRS FDRNSARFER GVGGILKHRG 
541:	RYLLIFALIT AGTGYLFTQI PKAFLPSEDQ GLMMTEVRMP LNASAERTEV VLQEVKDYLL 
601:	KEEGQLVDHV MTVNGFNFAG RGQNSGLVLV VLKDWAARQA AGEDVLSVAE RANARFARIK 
661:	DATVMAFVPP AVLEMGNAMG FDLYLQDNLG LGHESLMAAR NQFLELAAEN PSLRAVRPNG 
721:	KDDEPQFQVK IDDEKARALQ VSIASINDTM SAAWGSMYVN DFIDLGRVKR VYIQGVDSSR 
781:	IAPEDFDKWY VRNALGEMVP FSAFATGEWI HGSPKLERYG GISAVNILGE PAPGFSTGDA 
841:	MIAIAQIMQQ LPSGIGLSYN GLSYEEIRTG DQAPMLYALT VLIVFLCLAA LYESWSVPMS 
901:	VILVVPLGIF GAVLATLWRG LEADVYFQVG LMTTVGLSAK NAILIIEFAK ELYEKEGVPL 
961:	VKAAIEAARL RLRPIIMTSL AFTFGVLPMA RATGASAGSQ HSIATGVVSG MITATVLAVF 
1021:	FVPLFYVVVV KVFERNRKPA ALAEEELA