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1.A.1.11.22
The phosphoinositide (PI(3,5)P2)-activated Na+two pore+channel-1, TPC1 of endosomes and lysosomes (Wang et al. 2012). Previously thought, incorectly, according to Wang et al. (2012), to be an NAADP-activated two pore voltage-dependent calcium channel protein.  However, Cang et al. (2013), showed that TPC1 and TPC2 (TC# 1.A.1.11.19) together form an ATP-sensitive two-pore Na channel that senses the metabolic state of the cell.  The channel complex detects nutrient status, becomes constitutively open upon nutrient removal, and controls the lysosome''s membrane potential, pH stability, and amino acid homeostasis.  May be regulated by the HCLS-associated X-1 (HAX-1) protein (Lam et al. 2013).

Accession Number:Q9EQJ0
Protein Name:Two pore calcium channel protein 1
Length:817
Molecular Weight:94496.00
Species:Mus musculus (Mouse) [10090]
Number of TMSs:10
Location1 / Topology2 / Orientation3: Lysosome membrane1 / Multi-pass membrane protein2
Substrate Na+

Cross database links:

Genevestigator: Q9EQJ0
eggNOG: NOG280094
HEGENOM: HOG000006773
Entrez Gene ID: 252972   
Pfam: PF00520   
KEGG: mmu:252972   

Gene Ontology

GO:0010008 C:endosome membrane
GO:0016021 C:integral to membrane
GO:0005765 C:lysosomal membrane
GO:0072345 F:NAADP-sensitive calcium-release channel activity
GO:0005244 F:voltage-gated ion channel activity

References (4)

[1] “Positional cloning of the murine flavivirus resistance gene.”  Perelygin A.A.et.al.   12080145
[2] “The transcriptional landscape of the mammalian genome.”  Carninci P.et.al.   16141072
[3] “The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).”  The MGC Project Teamet.al.   15489334
[4] “Prediction of the coding sequences of mouse homologues of KIAA gene: III. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries.”  Okazaki N.et.al.   14621295

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MAVSLDDDVP LILTLDEAES APLPPSNSLG QEQLPSKNGG SHSIHNSQVP SLVSGADSPP 
61:	SSPTGHNWEM NYQEAAIYLQ EGQNNDKFFT HPKDARALAA YLFVHNHFFY MMELLTALLL 
121:	LLLSLCESPA VPVLKLHTYV HATLELFALM VVVFELCMKL RWLGFHTFVR HKRTMVKTSV 
181:	LVVQFIEAIV VLVRQTSHVR VTRALRCIFL VDCRYCGGVR RNLRQIFQSL PPFMDILLLL 
241:	LFFMIIFAIL GFYLFSTNPS DPYFSTLENS IVNLFVLLTT ANFPDVMMPS YSRNPWSCVF 
301:	FIVYLSIELY FIMNLLLAVV FDTFNDIEKH KFKSLLLHKR TAIQHAYGLL ASQRRPAGIS 
361:	YRQFEGLMRF YKPRMSARER FLTFKALNQS NTPLLSLKDF YDIYEVAALQ WKAKRNRQHW 
421:	FDELPRTAFL IFKGINILVN SKAFQYFMYL VVAVNGVWIL VETFMLKGGN FTSKHVPWSY 
481:	LVFLTIYGVE LFMKVAGLGP VEYLSSGWNL FDFSVTAFAF LGLLALTLNM EPFYFIVVLR 
541:	PLQLLRLFKL KKRYRNVLDT MFELLPRMAS LGLTLLTFYY SFAIVGMEFF NGRLTPNCCN 
601:	TSTVADAYRF INHTVGNKTK VEEGYYYLNN FDNILNSFVT LFELTVVNNW YIIMEGVTSQ 
661:	TSHWSRLYFM TFYIVTMVVM TIIVAFILEA FVFRMNYSRK SQDSEVDSGI VIEKEMSKEE 
721:	LMAVLELYRE ERGTSSDVTR LLDTLSQMEK YQQNSMVFLG RRSRTKSDLS LKMYQEEIQE 
781:	WYEEHAREQE QQKLRGSVPG PAAQQPPGSR QRSQTVT