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1.A.1.11.25
Endosomal/lysosomal, two pore Na+and Ca2+-release channel  (Na+> Ca2+) protein of 816 aas and 12 TMSs, TPC1 or TPCN1 (Guo et al. 2017).  Endosomes and lysosomes are electrically excitable organelles (Cang et al. 2014). In a subpopulation of endolysosomes, a brief electrical stimulus elicits a prolonged membrane potential depolarization spike. The organelles have a depolarization-activated, non-inactivating Na+ channel (lysoNaV). The channel is formed by a two-repeat six-transmembrane-spanning (2x6 TMS) protein, TPC1, which represents the evolutionary transition between 6 TMS and 4x6 TMS voltage-gated channels. Luminal alkalization also opens lysoNaV by markedly shifting the channel's voltage dependence of activation toward hyperpolarization. Thus, TPC1 is a voltage-gated Na+ channel that senses pH changes and confers electrical excitability to organelles (Cang et al. 2014). Essential for Ebola virus (EBOV) host entry.  Several inhibitors act in the nM (tetrandrine) or μM (verapamil; Ned19) range to block Na+ and Ca2+ channel activity, inhibit virus escape from membrane vesicles and may possibly be used to treat the disease (Sakurai et al. 2015).

Accession Number:Q9ULQ1
Protein Name:Two pore calcium channel protein 1
Length:816
Molecular Weight:94147.00
Species:Homo sapiens (Human) [9606]
Number of TMSs:10
Location1 / Topology2 / Orientation3: Lysosome membrane1 / Multi-pass membrane protein2
Substrate Ca2+

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FASTA formatted sequence
1:	MAVSLDDDVP LILTLDEGGS APLAPSNGLG QEELPSKNGG SYAIHDSQAP SLSSGGESSP 
61:	SSPAHNWEMN YQEAAIYLQE GENNDKFFTH PKDAKALAAY LFAHNHLFYL MELATALLLL 
121:	LLSLCEAPAV PALRLGIYVH ATLELFALMV VVFELCMKLR WLGLHTFIRH KRTMVKTSVL 
181:	VVQFVEAIVV LVRQMSHVRV TRALRCIFLV DCRYCGGVRR NLRQIFQSLP PFMDILLLLL 
241:	FFMIIFAILG FYLFSPNPSD PYFSTLENSI VSLFVLLTTA NFPDVMMPSY SRNPWSCVFF 
301:	IVYLSIELYF IMNLLLAVVF DTFNDIEKRK FKSLLLHKRT AIQHAYRLLI SQRRPAGISY 
361:	RQFEGLMRFY KPRMSARERY LTFKALNQNN TPLLSLKDFY DIYEVAALKW KAKKNREHWF 
421:	DELPRTALLI FKGINILVKS KAFQYFMYLV VAVNGVWILV ETFMLKGGNF FSKHVPWSYL 
481:	VFLTIYGVEL FLKVAGLGPV EYLSSGWNLF DFSVTVFAFL GLLALALNME PFYFIVVLRP 
541:	LQLLRLFKLK ERYRNVLDTM FELLPRMASL GLTLLIFYYS FAIVGMEFFC GIVFPNCCNT 
601:	STVADAYRWR NHTVGNRTVV EEGYYYLNNF DNILNSFVTL FELTVVNNWY IIMEGVTSQT 
661:	SHWSRLYFMT FYIVTMVVMT IIVAFILEAF VFRMNYSRKN QDSEVDGGIT LEKEISKEEL 
721:	VAVLELYREA RGASSDVTRL LETLSQMERY QQHSMVFLGR RSRTKSDLSL KMYQEEIQEW 
781:	YEEHAREQEQ QRQLSSSAAP AAQQPPGSRQ RSQTVT