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1.A.1.13.1
6TMS K+ channel (Kuo et al., 2003).  The kch gene, the only known potassium channel gene in E. coli, has the property to express both full-length Kch and its cytosolic domain (RCK) due to a methionine at position 240. The RCK domains form an octameric ring structure and regulate the gating of the potassium channels after having bound certain ligands. Several different gating ring structures have been reported for the soluble RCK domains.  The octameric structure of Kch may be composed of two tetrameric full-length proteins through RCK interaction (Kuang et al. 2013).

Accession Number:P31069
Protein Name:KCH aka B1250
Length:417
Molecular Weight:46063.00
Species:Escherichia coli [83333]
Number of TMSs:7
Location1 / Topology2 / Orientation3: Cell inner membrane1 / Multi-pass membrane protein2
Substrate K+

Cross database links:

Genevestigator: P31069
EchoBASE: EB1563
EcoGene: EG11606
eggNOG: COG1226
DIP: DIP-10055N
RefSeq: AP_001876.1    NP_415766.1   
Entrez Gene ID: 945841   
Pfam: PF07885    PF02254   
BioCyc: EcoCyc:KCH-MONOMER    ECOL168927:B1250-MONOMER   
KEGG: ecj:JW1242    eco:b1250   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0005488 F:binding
GO:0003824 F:catalytic activity
GO:0005249 F:voltage-gated potassium channel activity
GO:0008152 P:metabolic process
GO:0006813 P:potassium ion transport

References (6)

[1] “An Escherichia coli homologue of eukaryotic potassium channel proteins.”  Milkman R.et.al.   8170937
[2] “A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map.”  Aiba H.et.al.   9097039
[3] “The complete genome sequence of Escherichia coli K-12.”  Blattner F.R.et.al.   9278503
[4] “Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.”  Hayashi K.et.al.   16738553
[5] “Global topology analysis of the Escherichia coli inner membrane proteome.”  Daley D.O.et.al.   15919996
[6] “Structure of the RCK domain from the E. coli K+ channel and demonstration of its presence in the human BK channel.”  Jiang Y.et.al.   11301020
Structure:
1ID1     

External Searches:

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  • 2° Structure (Network Protein Sequence Analysis)

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MSHWATFKQT ATNLWVTLRH DILALAVFLN GLLIFKTIYG MSVNLLDIFH IKAFSELDLS 
61:	LLANAPLFML GVFLVLNSIG LLFRAKLAWA ISIILLLIAL IYTLHFYPWL KFSIGFCIFT 
121:	LVFLLILRKD FSHSSAAAGT IFAFISFTTL LFYSTYGALY LSEGFNPRIE SLMTAFYFSI 
181:	ETMSTVGYGD IVPVSESARL FTISVIISGI TVFATSMTSI FGPLIRGGFN KLVKGNNHTM 
241:	HRKDHFIVCG HSILAINTIL QLNQRGQNVT VISNLPEDDI KQLEQRLGDN ADVIPGDSND 
301:	SSVLKKAGID RCRAILALSD NDADNAFVVL SAKDMSSDVK TVLAVSDSKN LNKIKMVHPD 
361:	IILSPQLFGS EILARVLNGE EINNDMLVSM LLNSGHGIFS DNDELETKAD SKESAQK