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1.A.1.14.1
Voltage-activated, Ca2+  channel blocker-inhibited, Na channel, NaChBac (Ren et al., 2001; Zhao et al., 2004Nurani et al, 2008; Charalambous and Wallace, 2011). Transmembrane and extramembrane contributions to thermal stability have been studied (Powl et al., 2012). Deprotonation of arginines in S4 is involved in NaChBac gating (Paldi, 2012). Hinge-bending motions in the pore domain of NaChBac have been reported (Barber et al., 2012).  The C-terminal coiled-coli stabilizes subunit interactions (Mio et al. 2010).  Within the 4 TMS voltage sensor, coupling between residues in S1 and S4 determines its resting conformation (Paldi and Gurevitz 2010). The conserved asparagine was changed to aspartate, N225D, and this substitution shifted the voltage-dependence of inactivation by 25 mV to more hyperpolarized potentials. The mutant also displays greater thermostability (O'Reilly et al. 2017).  Possibly, the side-chain amido group of asn 225 forms one or more hydrogen bonds with different channel elements and that these interactions are important for normal channel function.

Accession Number:Q9KCR8
Protein Name:NaChBac aka BH1501
Length:274
Molecular Weight:31456.00
Species:Bacillus halodurans [86665]
Number of TMSs:5
Location1 / Topology2 / Orientation3: Mitochondrion outer membrane1 / Multi-pass membrane protein2
Substrate Ca2+

Cross database links:

HEGENOM: HBG320980
RefSeq: NP_242367.1   
Entrez Gene ID: 890883   
Pfam: PF00520   
BioCyc: BHAL272558:BH1501-MONOMER   
KEGG: bha:BH1501   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005216 F:ion channel activity
GO:0006811 P:ion transport
GO:0055085 P:transmembrane transport

References (1)

[1] “Complete genome sequence of the alkaliphilic bacterium Bacillus halodurans and genomic sequence comparison with Bacillus subtilis.”  Takami H.et.al.   11058132

External Searches:

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  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MKMEARQKQN SFTSKMQKIV NHRAFTFTVI ALILFNALIV GIETYPRIYA DHKWLFYRID 
61:	LVLLWIFTIE IAMRFLASNP KSAFFRSSWN WFDFLIVAAG HIFAGAQFVT VLRILRVLRV 
121:	LRAISVVPSL RRLVDALVMT IPALGNILIL MSIFFYIFAV IGTMLFQHVS PEYFGNLQLS 
181:	LLTLFQVVTL ESWASGVMRP IFAEVPWSWL YFVSFVLIGT FIIFNLFIGV IVNNVEKAEL 
241:	TDNEEDGEAD GLKQEISALR KDVAELKSLL KQSK