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1.A.1.17.1
The archaeal voltage-regulated K channel, KvAP (Ruta et al., 2003). X-ray and solution structures are available. The latter shows phospholipid interactions with the isolated voltage sensor domain (Butterwick and MacKinnon 2010; Li et al. 2014). The gating-charge arginine in TMS4 of the voltage sensor forms part of the helical hairpin "paddle", and it moves 15-20 Å through the membrane to open the pore (Ruta et al., 2005). The orientation and depth of insertion of the voltage-sensing S4 helix has been determined (Doherty et al., 2010). A synthetic S6 segment derived from the KvAP channel self-assembles, permeabilizes lipid vesicles, and exhibits ion channel activity in bilayer lipid membrane (Verma et al., 2011).  Thus the gating mechanism combines structural rearrangements and electric-field remodeling ( Li et al. 2014).  KvAP has been reconstituted in Giant Unilamellar Vesicles (GUVs) (Garten et al. 2015).  TMS4 (S4) which senses voltage also promotes membrane insertion of the voltage-sensor domain (Mishima et al. 2016). KvAP has a configuration consistent with a water channel, possibly underlying the conductance of protons, and other cations, through voltage-sensor domains (Freites et al. 2006).

Accession Number:Q9YDF8
Protein Name:KvAP
Length:295
Molecular Weight:32535.00
Species:Aeropyrum pernix [56636]
Number of TMSs:5
Location1 / Topology2 / Orientation3: Cell membrane1 / Multi-pass membrane protein2
Substrate K+

Cross database links:

DIP: DIP-29648N
RefSeq: NP_147625.1   
Entrez Gene ID: 1445031   
Pfam: PF07885   
BioCyc: APER272557:APE0955-MONOMER   
KEGG: ape:APE_0955   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0005267 F:potassium channel activity
GO:0005244 F:voltage-gated ion channel activity
GO:0006813 P:potassium ion transport

References (3)

[1] “Complete genome sequence of an aerobic hyper-thermophilic crenarchaeon, Aeropyrum pernix K1.”  Kawarabayasi Y.et.al.   10382966
[2] “Functional analysis of an archaebacterial voltage-dependent K+ channel.”  Ruta V.et.al.   12629550
[3] “X-ray structure of a voltage-dependent K(+) channel.”  Jiang Y.et.al.   12721618
Structure:
1ORQ   1ORS   2A0L   2KYH     

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MSVERWVFPG CSVMARFRRG LSDLGGRVRN IGDVMEHPLV ELGVSYAALL SVIVVVVEYT 
61:	MQLSGEYLVR LYLVDLILVI ILWADYAYRA YKSGDPAGYV KKTLYEIPAL VPAGLLALIE 
121:	GHLAGLGLFR LVRLLRFLRI LLIISRGSKF LSAIADAADK IRFYHLFGAV MLTVLYGAFA 
181:	IYIVEYPDPN SSIKSVFDAL WWAVVTATTV GYGDVVPATP IGKVIGIAVM LTGISALTLL 
241:	IGTVSNMFQK ILVGEPEPSC SPAKLAEMVS SMSEEEFEEF VRTLKNLRRL ENSMK