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1.A.1.20.4
K+ voltage-gated channel, rEAG1; Kv10.1; rat ether a go-go channel 1 (962 aas). Blocked by Cs+, Ba2+ and quinidine (Schwarzer et al., 2008). Cysteines control the N- and C-linker-dependent gating of KCNH1 potassium channels  (Sahoo et al., 2012).  The 3-d structure has been determined at 3.8 Å resolution using single-particle cryo-EM with calmodulin bound. The structure suggests a novel mechanism of voltage-dependent gating. Calmodulin binding closes the potassium pore (Whicher and MacKinnon 2016).

Accession Number:Q63472
Protein Name:Potassium voltage-gated channel subfamily H member 1
Length:962
Molecular Weight:108291.00
Species:Rattus norvegicus (Rat) [10116]
Number of TMSs:7
Location1 / Topology2 / Orientation3: Membrane1 / Multi-pass membrane protein2
Substrate K+

Cross database links:

Genevestigator: Q63472
eggNOG: roNOG06149
RefSeq: NP_113930.1   
Entrez Gene ID: 65198   
Pfam: PF00027    PF00520    PF00989   
KEGG: rno:65198   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005516 F:calmodulin binding
GO:0000155 F:two-component sensor activity
GO:0005249 F:voltage-gated potassium channel activity
GO:0006813 P:potassium ion transport
GO:0006355 P:regulation of transcription, DNA-dependent
GO:0007165 P:signal transduction
GO:0055085 P:transmembrane transport
GO:0000160 P:two-component signal transduction system (p...

References (4)

[1] “Functional expression of a rat homologue of the voltage gated ether a go-go potassium channel reveals differences in selectivity and activation kinetics between the Drosophila channel and its mammalian counterpart.”  Ludwig J.et.al.   7925287
[2] “Carboxy-terminal domain mediates assembly of the voltage-gated rat ether-a-go-go potassium channel.”  Ludwig J.et.al.   9400421
[3] “KCR1, a membrane protein that facilitates functional expression of non-inactivating K+ currents associates with rat EAG voltage-dependent K+ channels.”  Hoshi N.et.al.   9722534
[4] “Expression of mRNA for voltage-dependent and inward-rectifying K channels in GH3/B6 cells and rat pituitary.”  Wulfsen I.et.al.   10718922
Structure:
5k7l     

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MTMAGGRRGL VAPQNTFLEN IVRRSNDTNF VLGNAQIVDW PIVYSNDGFC KLSGYHRAEV 
61:	MQKSSACSFM YGELTDKDTV EKVRQTFENY EMNSFEILMY KKNRTPVWFF VKIAPIRNEQ 
121:	DKVVLFLCTF SDITAFKQPI EDDSCKGWGK FARLTRALTS SRGVLQQLAP SVQKGENVHK 
181:	HSRLAEVLQL GSDILPQYKQ EAPKTPPHII LHYCVFKTTW DWIILILTFY TAILVPYNVS 
241:	FKTRQNNVAW LVVDSIVDVI FLVDIVLNFH TTFVGPAGEV ISDPKLIRMN YLKTWFVIDL 
301:	LSCLPYDVIN AFENVDEGIS SLFSSLKVVR LLRLGRVARK LDHYIEYGAA VLVLLVCVFG 
361:	LAAHWMACIW YSIGDYEIFD EDTKTIRNNS WLYQLALDIG TPYQFNGSGS GKWEGGPSKN 
421:	SVYISSLYFT MTSLTSVGFG NIAPSTDIEK IFAVAIMMIG SLLYATIFGN VTTIFQQMYA 
481:	NTNRYHEMLN SVRDFLKLYQ VPKGLSERVM DYIVSTWSMS RGIDTEKVLQ ICPKDMRADI 
541:	CVHLNRKVFK EHPAFRLASD GCLRALAMEF QTVHCAPGDL IYHAGESVDS LCFVVSGSLE 
601:	VIQDDEVVAI LGKGDVFGDV FWKEATLAQS CANVRALTYC DLHVIKRDAL QKVLEFYTAF 
661:	SHSFSRNLIL TYNLRKRIVF RKISDVKREE EERMKRKNEA PLILPPDHPV RRLFQRFRQQ 
721:	KEARLAAERG GRDLDDLDVE KGNALTDHTS ANHSLVKASV VTVRESPATP VSFQAASTST 
781:	VSDHAKLHAP GSECLGPKAG GGDPAKRKGW ARFKDACGKG EDWNKVSKAE SMETLPERTK 
841:	ASGEATLKKT DSCDSGITKS DLRLDNVGEA RSPQDRSPIL AEVKHSFYPI PEQTLQATVL 
901:	EVKHELKEDI KALNAKMTSI EKQLSEILRI LMSRGSSQSP QDTCEVSRPQ SPESDRDIFG 
961:	AS