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1.A.1.25.1
The 6TMS bacterial cyclic nucleotide-regulated, voltage independent channel, MlotiK1 or MloK1 (Clayton et al., 2008). Gating involves large rearrangements of the cyclic nucleotide-binding domains (Mari et al., 2011).  The electron crystalographic structure is available (PDB 4CHW)revealing ligand-induced structural changes (Schünke et al. 2011; Scherer et al. 2014; Kowal et al. 2014). Such changes may be lipid dependent (Scherer et al. 2014; Kowal et al. 2014). Such changes may be lipid dependent (McCoy et al. 2014).

Accession Number:Q98GN8
Protein Name:MlotiK1
Length:355
Molecular Weight:37735.00
Species:Rhizobium loti (Mesorhizobium loti) [381]
Number of TMSs:6
Location1 / Topology2 / Orientation3: Cell membrane1 / Multi-pass membrane protein2
Substrate Unknown

Cross database links:

HEGENOM: HBG299487
DIP: DIP-29507N
RefSeq: NP_104392.1   
Entrez Gene ID: 1227053   
Pfam: PF00027    PF07885   
Drugbank: Drugbank Link   
KEGG: mlo:mll3241   

Gene Ontology

GO:0008076 C:voltage-gated potassium channel complex
GO:0030552 F:cAMP binding
GO:0005249 F:voltage-gated potassium channel activity
GO:0006813 P:potassium ion transport

References (3)

[1] “Complete genome structure of the nitrogen-fixing symbiotic bacterium Mesorhizobium loti.”  Kaneko T.et.al.   11214968
[2] “Structural basis of ligand activation in a cyclic nucleotide regulated potassium channel.”  Clayton G.M.et.al.   15550244
[3] “Structure of the transmembrane regions of a bacterial cyclic nucleotide-regulated channel.”  Clayton G.M.et.al.   18216238
Structure:
1U12   1VP6   2K0G   2ZD9   3BEH   3CL1   3CLP   3CO2   2KXL   4CHV   [...more]

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MSVLPFLRIY APLNAVLAAP GLLAVAALTI PDMSGRSRLA LAALLAVIWG AYLLQLAATL 
61:	LKRRAGVVRD RTPKIAIDVL AVLVPLAAFL LDGSPDWSLY CAVWLLKPLR DSTFFPVLGR 
121:	VLANEARNLI GVTTLFGVVL FAVALAAYVI ERDIQPEKFG SIPQAMWWAV VTLSTTGYGD 
181:	TIPQSFAGRV LAGAVMMSGI GIFGLWAGIL ATGFYQEVRR GDFVRNWQLV AAVPLFQKLG 
241:	PAVLVEIVRA LRARTVPAGA VICRIGEPGD RMFFVVEGSV SVATPNPVEL GPGAFFGEMA 
301:	LISGEPRSAT VSAATTVSLL SLHSADFQML CSSSPEIAEI FRKTALERRG AAASA