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1.A.1.5.2
Hyperpolarization-activated and cyclic nucleotide-gated K+ channel, HCN (bCNG-1) (PNa+/PK+ ≈ 0.3). The human orthologue (O88703) is 863 aas in length and catalyzes mixed monovalent cation currents K+:Na+= 4:1 (Lyashchenko and Tibbs et al., 2008). Biel et al. (2009) present a detailed review of hyperpolarization-activated cation-channels. Inhibited by nicotine and epibatidine which bind to the inner pore (Griguoli et al., 2010).

Accession Number:O88704
Protein Name:Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1
Length:910
Molecular Weight:102432.00
Species: [10090]
Number of TMSs:3
Location1 / Topology2 / Orientation3: Membrane1 / Multi-pass membrane protein2
Substrate K+

Cross database links:

Genevestigator: O88704
eggNOG: roNOG13745
HEGENOM: HBG447083
RefSeq: NP_034538.2   
Entrez Gene ID: 15165   
Pfam: PF00027    PF00520    PF08412   
KEGG: mmu:15165   

Gene Ontology

GO:0030424 C:axon
GO:0030425 C:dendrite
GO:0016021 C:integral to membrane
GO:0030552 F:cAMP binding
GO:0005515 F:protein binding
GO:0005272 F:sodium channel activity
GO:0005249 F:voltage-gated potassium channel activity
GO:0045176 P:apical protein localization
GO:0006813 P:potassium ion transport
GO:0006814 P:sodium ion transport
GO:0055085 P:transmembrane transport

References (11)

[1] “Interactive cloning with the SH3 domain of N-src identifies a new brain specific ion channel protein, with homology to eag and cyclic nucleotide-gated channels.”  Santoro B.et.al.   9405696
[2] “A family of hyperpolarization-activated cation channels.”  Ludwig A.et.al.   9634236
[3] “The transcriptional landscape of the mammalian genome.”  Carninci P.et.al.   16141072
[4] “Identification of a gene encoding a hyperpolarization-activated 'pacemaker' channel of brain.”  Santoro B.et.al.   9630217
[5] “MinK-related peptide 1: a beta subunit for the HCN ion channel subunit family enhances expression and speeds activation.”  Yu H.et.al.   11420311
[6] “Molecular mechanism of cAMP modulation of HCN pacemaker channels.”  Wainger B.J.et.al.   11459060
[7] “Hyperpolarization-activated channels HCN1 and HCN4 mediate responses to sour stimuli.”  Stevens D.R.et.al.   11675786
[8] “Dominant-negative suppression of HCN1- and HCN2-encoded pacemaker currents by an engineered HCN1 construct: insights into structure-function relationships and multimerization.”  Xue T.et.al.   12089064
[9] “Different roles for the cyclic nucleotide binding domain and amino terminus in assembly and expression of hyperpolarization-activated, cyclic nucleotide-gated channels.”  Proenza C.et.al.   12034718
[10] “An external determinant in the S5-P linker of the pacemaker (HCN) channel identified by sulfhydryl modification.”  Xue T.et.al.   12351622
[11] “Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations.”  Munton R.P.et.al.   17114649
Structure:
3U0Z     

External Searches:

  • Search: DB with
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  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MEGGGKPNSA SNSRDDGNSV FPSKAPATGP VAADKRLGTP PGGGAAGKEH GNSVCFKVDG 
61:	GGGEEPAGSF EDAEGPRRQY GFMQRQFTSM LQPGVNKFSL RMFGSQKAVE KEQERVKTAG 
121:	FWIIHPYSDF RFYWDLIMLI MMVGNLVIIP VGITFFTEQT TTPWIIFNVA SDTVFLLDLI 
181:	MNFRTGTVNE DSSEIILDPK VIKMNYLKSW FVVDFISSIP VDYIFLIVEK GMDSEVYKTA 
241:	RALRIVRFTK ILSLLRLLRL SRLIRYIHQW EEIFHMTYDL ASAVVRIFNL IGMMLLLCHW 
301:	DGCLQFLVPL LQDFPPDCWV SLNEMVNDSW GKQYSYALFK AMSHMLCIGY GAQAPVSMSD 
361:	LWITMLSMIV GATCYAMFVG HATALIQSLD SSRRQYQEKY KQVEQYMSFH KLPADMRQKI 
421:	HDYYEHRYQG KIFDEENILS ELNDPLREEI VNFNCRKLVA TMPLFANADP NFVTAMLSKL 
481:	RFEVFQPGDY IIREGAVGKK MYFIQHGVAG VITKSSKEMK LTDGSYFGEI CLLTKGRRTA 
541:	SVRADTYCRL YSLSVDNFNE VLEEYPMMRR AFETVAIDRL DRIGKKNSIL LQKFQKDLNT 
601:	GVFNNQENEI LKQIVKHDRE MVQAIPPINY PQMTALNCTS STTTPTSRMR TQSPPVYTAT 
661:	SLSHSNLHSP SPSTQTPQPS AILSPCSYTT AVCSPPIQSP LATRTFHYAS PTASQLSLMQ 
721:	QPQQQLPQSQ VQQTQTQTQQ QQQQQQQQQQ QQQQQQQQQQ QQQQQQQQQQ QQQQQPQTPG 
781:	SSTPKNEVHK STQALHNTNL TKEVRPLSAS QPSLPHEVST LISRPHPTVG ESLASIPQPV 
841:	AAVHSTGLQA GSRSTVPQRV TLFRQMSSGA IPPNRGVPPA PPPPAAVQRE SPSVLNTDPD 
901:	AEKPRFASNL