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1.A.11.1.8
AMT of 514 aas and 11 TMSs. Trypanosoma cruzi, the etiologic agent of Chagas disease, undergoes drastic metabolic changes when it transits between a vector and mammalian hosts. Amino acid catabolism leads to the production of NH4+, which must be detoxified. Cruz-Bustos et al. 2018 identified an intracellular ammonium transporter of T. cruzi (TcAMT) that localizes to acidic compartments (reservosomes, lysosomes). TcAMT possesses all conserved and functionally important residues that form the pore in other ammonium transporters. Functional expression in Xenopus oocytes followed by a two-electrode voltage clamp showed an inward current that is NH4+ dependent at a resting membrane potential lower than -120 mV and is not pH dependent, suggesting that TcAMT is an NH4+or NH3/H+ transporter. Ablation of TcAMT resulted in defects in epimastigote and amastigote replication, differentiation, and resistance to starvation and osmotic stress (Cruz-Bustos et al. 2018). 

Accession Number:Q4DC59
Protein Name:Ammonium transporter
Length:514
Molecular Weight:55505.00
Species:Trypanosoma cruzi (strain CL Brener) [353153]
Number of TMSs:11
Location1 / Topology2 / Orientation3: Membrane1 / Multi-pass membrane protein2
Substrate

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MSSGASTEGK CLPEDSDISW VLISSVLVLG MMPGLGFFEA GLLRSKNTTS VFAQIFSGCA 
61:	VLSVLWVCAG YSLTMGRSAG GKGIIGTFRR AFMMNVDYNT CYGGTVIPEA LFAFFQMMFA 
121:	TITPLLMTGA YAERLAFRPF LFFTILWEII VYFFVAHWVW APEGWMRGMG VQDFAGGIVI 
181:	HVTAGVSSLV CAVVLGRRRD FHIHRGEAPY SSLPLTCIGA TMLWTGWFGF NGGSALQSGK 
241:	GAVYAVINSQ VAAAVCSCCF LFFHMLRTKK ASLIAMINGA IAGLAGITPT SGYITVPSSI 
301:	ICAFFIAVFA TVSVYLIKHK LRIDDALDVS SIHGVPGLVG AVFIGFSGSS AVGGADGLLY 
361:	GGGIRLLGLQ CLGCIVAATW AGFWTFVILL IIGRFYRLRV TDEQEHHGLD HGQHSEVAWI 
421:	LQASNVGGDA LPIKNRKRVK QVRNSSLFIG ETGTYIVEKE RGDSILVVER VNNDEVDIDD 
481:	DPREEGFNTM TLAVNADARN EITTDIDERL INCS