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1.A.2.2.1
Prokaryotic K+-selective Kir channel KirBac1.1 (selectivity: K+ = Rb+ = Cs+ >> Li+, Na+ or NMGM) (Enkvetchakul et al., 2004), inward rectifying (Cheng et al., 2009). Closure of the Kir1.1 pH gate results from steric occlusion of the permeation path by the convergence of four leucines (or phenylalanines) at the cytoplasmic apex of the inner transmembrane helices. In the open state, K+ crosses the pH gate together with its hydration shell (Sackin et al. 2005). The inhibitory cholesterol binding site has been identified (Fürst et al. 2014). Conformational changes associated with an open activation gate have been identified, and these suggest a unique allosteric pathway that ties the selectivity filter to the activation gate through interactions between both transmembrane helices, the turret, the selectivity filter loop, and the pore helix. Specific residues involved in this conformational exchange that are highly conserved among human Kir channels have also been identified (Amani et al. 2020).

Accession Number:P83698
Protein Name:Inward rectifier potassium channel protein
Length:333
Molecular Weight:37133.00
Species:Burkholderia pseudomallei (Pseudomonas pseudomallei) [28450]
Number of TMSs:3
Location1 / Topology2 / Orientation3: Apical cell membrane1 / Multi-pass membrane protein2
Substrate K+

Cross database links:

HEGENOM: HBG480115
RefSeq: YP_106981.1   
Entrez Gene ID: 3094088   
Pfam: PF01007   
BioCyc: BPSE272560:BPSL0355-MONOMER   
KEGG: bps:BPSL0355   

Gene Ontology

GO:0016020 C:membrane
GO:0005242 F:inward rectifier potassium channel activity
GO:0006813 P:potassium ion transport

References (2)

[1] “Crystal structure of the potassium channel KirBac1.1 in the closed state.”  Kuo A.et.al.   12738871
[2] “Genomic plasticity of the causative agent of melioidosis, Burkholderia pseudomallei.”  Holden M.T.G.et.al.   15377794
Structure:
1P7B   2WLL     

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MNVDPFSPHS SDSFAQAASP ARKPPRGGRR IWSGTREVIA YGMPASVWRD LYYWALKVSW 
61:	PVFFASLAAL FVVNNTLFAL LYQLGDAPIA NQSPPGFVGA FFFSVETLAT VGYGDMHPQT 
121:	VYAHAIATLE IFVGMSGIAL STGLVFARFA RPRAKIMFAR HAIVRPFNGR MTLMVRAANA 
181:	RQNVIAEARA KMRLMRREHS SEGYSLMKIH DLKLVRNEHP IFLLGWNMMH VIDESSPLFG 
241:	ETPESLAEGR AMLLVMIEGS DETTAQVMQA RHAWEHDDIR WHHRYVDLMS DVDGMTHIDY 
301:	TRFNDTEPVE PPGAAPDAQA FAAKPGEGDA RPV