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1.A.35.2.1
Aluminum resistance protein AlR1p; Alr1; ALR1, LLR1; MNR1 (Mg2+ homeostasis transporter [Mg2+ regulated]) AlR1p and AlR2p (P43553; a close paralogue) both catalyze uptake of Mg2+ and a variety of heavy metals (da Costa et al., 2007). Truncation of Alr1 showed that the N-terminal 239 amino acids and the C-terminal 53 amino acids are not essential for magnesium uptake (Lee and Gardner 2006). Mutations in the C-terminal part of ALR1 that is homologous to bacterial CorA magnesium transporters that gave severe phenotypes had amino acid changes in the small region containing the TMSs. Eighteen single amino acid mutants in this region were classified into three categories for magnesium uptake: no, low and moderate activity. Conservative mutations that reduced or inactivated uptake led to the identification of Ser(729), Ile(746) and Met(762) (part of the conserved GMN motif) as critical residues. High expression of inactive mutants inhibited the capability of wild-type Alr1 to transport magnesium, consistent with Alr1 forming homo-oligomers (Lee and Gardner 2006).

Accession Number:Q08269
Protein Name:ALR1 aka YOL130W
Length:859
Molecular Weight:95869.00
Species:Saccharomyces cerevisiae (Baker's yeast) [4932]
Number of TMSs:2
Location1 / Topology2 / Orientation3: Membrane1 / Multi-pass membrane protein2
Substrate Al3+, Mg2+

Cross database links:

Genevestigator: Q08269
eggNOG: fuNOG05873
HEGENOM: HBG396603
DIP: DIP-1184N
RefSeq: NP_014511.1   
Entrez Gene ID: 853990   
Pfam: PF01544   
KEGG: sce:YOL130W   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0015082 F:di-, tri-valent inorganic cation transmembr...
GO:0046873 F:metal ion transmembrane transporter activity
GO:0005515 F:protein binding
GO:0015693 P:magnesium ion transport
GO:0055085 P:transmembrane transport

References (8)

[1] “Sequence analysis of a 13.4 kbp fragment from the left arm of chromosome XV reveals a malate dehydrogenase gene, a putative Ser/Thr protein kinase, the ribosomal L25 gene and four new open reading frames.”  Casamayor A.et.al.   8896265
[2] “The nucleotide sequence of Saccharomyces cerevisiae chromosome XV.”  Dujon B.et.al.   9169874
[3] “Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae.”  Hu Y.et.al.   17322287
[4] “Overexpression of the Saccharomyces cerevisiae magnesium transport system confers resistance to aluminum ion.”  MacDiarmid C.W.et.al.   9430719
[5] “A proteomics approach to understanding protein ubiquitination.”  Peng J.et.al.   12872131
[6] “Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae.”  Li X.et.al.   17330950
[7] “Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases.”  Smolka M.B.et.al.   17563356
[8] “A multidimensional chromatography technology for in-depth phosphoproteome analysis.”  Albuquerque C.P.et.al.   18407956

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MSSSSSSSES SPNLSRSNSL ANTMVSMKTE DHTGLYDHRQ HPDSLPVRHQ PPTLKNKEIA 
61:	KSTKPSIPKE QKSATRYNSH VDVGSVPSRG RMDFEDEGQG MDETVAHHQL RASAILTSNA 
121:	RPSRLAHSMP HQRQLYVESN IHTPPKDVGV KRDYTMSSST ASSGNKSKLS ASSSASPITK 
181:	VRKSSLVSPV LEIPHESKSD THSKLAKPKK RTYSTTSAHS SINPAVLLTK STSQKSDADD 
241:	DTLERKPVRM NTRASFDSDV SQASRDSQET EEDVCFPMPP QLHTRVNGID FDELEEYAQF 
301:	ANAEKSQFLA SLQVPNEQKY SNVSQDIGFT SSTSTSGSSA ALKYTPRVSQ TGEKSESTNE 
361:	TEIHEKKEDE HEKIKPSLHP GISFGKNKVE GEENENIPSN DPAYCSYQGT DFQIPNRFSF 
421:	FCSESDETVH ASDIPSLVSE GQTFYELFRG GEPTWWLDCS CPTDDEMRCI AKAFGIHPLT 
481:	AEDIRMQETR EKVELFKSYY FVCFHTFEND KESEDFLEPI NVYIVVCRSG VLTFHFGPIS 
541:	HCANVRRRVR QLRDYVNVNS DWLCYALIDD ITDSFAPVIQ SIEYEADAIE DSVFMARDMD 
601:	FAAMLQRIGE SRRKTMTLMR LLSGKADVIK MFAKRCQDEA NGIGPALTSQ INIANLQARQ 
661:	DNASHIKNNS STTVPNNYAP TTSQPRGDIA LYLGDIQDHL LTMFQNLLAY EKIFSRSHTN 
721:	YLAQLQVESF NSNNKVTEML GKVTMIGTML VPLNVITGLF GMNVKVPGEN SSIAWWFGIL 
781:	GVLLLLAVLG WFLASYWIKR IDPPATLNEA AESGAKSVIS SFLPKRNKRF NDRSKNINVR 
841:	AGPSNKSVAS LPSRYSRYD