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1.A.8.10.3
Tonoplast intrinsic protein 1.1 (permeable to water and H2O2)

Accession Number:P25818
Protein Name:Aquaporin TIP1.1 aka Aqp.1
Length:251
Molecular Weight:25620.00
Species:Arabidopsis thaliana (Mouse-ear cress) [3702]
Number of TMSs:7
Location1 / Topology2 / Orientation3: Vacuole membrane1 / Multi-pass membrane protein2
Substrate water, H2O2

Cross database links:

Genevestigator: P25818
eggNOG: KOG0223
HEGENOM: HBG705794
RefSeq: NP_181221.1   
Entrez Gene ID: 818255   
Pfam: PF00230   
KEGG: ath:AT2G36830   

Gene Ontology

GO:0042807 C:central vacuole
GO:0009941 C:chloroplast envelope
GO:0016021 C:integral to membrane
GO:0009705 C:plant-type vacuole membrane
GO:0005886 C:plasma membrane
GO:0000326 C:protein storage vacuole
GO:0015204 F:urea transmembrane transporter activity
GO:0015250 F:water channel activity
GO:0055085 P:transmembrane transport
GO:0015840 P:urea transport
GO:0006833 P:water transport

References (14)

[1] “Root-specific genes from tobacco and Arabidopsis homologous to an evolutionarily conserved gene family of membrane channel proteins.”  Yamamoto Y.T.et.al.   2129561
[2] “Vegetative and seed-specific forms of tonoplast intrinsic protein in the vacuolar membrane of Arabidopsis thaliana.”  Hoefte H.R.et.al.   16668923
[3] “Cloning of two gibberellin-regulated cDNAs from Arabidopsis thaliana by subtractive hybridization: expression of the tonoplast water channel, gamma-TIP, is increased by GA3.”  Phillips A.L.et.al.   8155880
[4] “Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.”  Lin X.et.al.   10617197
[5] “Empirical analysis of transcriptional activity in the Arabidopsis genome.”  Yamada K.et.al.   14593172
[6] “An inventory of 1152 expressed sequence tags obtained by partial sequencing of cDNAs from Arabidopsis thaliana.”  Hoefte H.et.al.   8281187
[7] “Further progress towards a catalogue of all Arabidopsis genes: analysis of a set of 5000 non-redundant ESTs.”  Cooke R.et.al.   8580968
[8] “The vacuolar membrane protein gamma-TIP creates water specific channels in Xenopus oocytes.”  Maurel C.et.al.   8508761
[9] “Characterization of a new vacuolar membrane aquaporin sensitive to mercury at a unique site.”  Daniels M.J.et.al.   8624437
[10] “From genome to function: the Arabidopsis aquaporins.”  Quigley F.et.al.   11806824
[11] “Urea transport by nitrogen-regulated tonoplast intrinsic proteins in Arabidopsis.”  Liu L.-H.et.al.   14576283
[12] “Loss of TIP1;1 aquaporin in Arabidopsis leads to cell and plant death.”  Ma S.et.al.   15584951
[13] “Arabidopsis tonoplast proteins TIP1 and TIP2 interact with the cucumber mosaic virus 1a replication protein.”  Kim M.J.et.al.   17030879
[14] “Specific aquaporins facilitate the diffusion of hydrogen peroxide across membranes.”  Bienert G.P.et.al.   17105724

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MPIRNIAIGR PDEATRPDAL KAALAEFIST LIFVVAGSGS GMAFNKLTEN GATTPSGLVA 
61:	AAVAHAFGLF VAVSVGANIS GGHVNPAVTF GAFIGGNITL LRGILYWIAQ LLGSVVACLI 
121:	LKFATGGLAV PAFGLSAGVG VLNAFVFEIV MTFGLVYTVY ATAIDPKNGS LGTIAPIAIG 
181:	FIVGANILAG GAFSGASMNP AVAFGPAVVS WTWTNHWVYW AGPLVGGGIA GLIYEVFFIN 
241:	TTHEQLPTTD Y