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1.A.8.10.6
The pollen-specific water/urea aquaporin, Tip1;3 (Soto et al. 2008)

Accession Number:O82598
Protein Name:Aquaporin TIP1-3
Length:252
Molecular Weight:25914.00
Species:Arabidopsis thaliana (Mouse-ear cress) [3702]
Number of TMSs:6
Location1 / Topology2 / Orientation3: Vacuole membrane1 / Multi-pass membrane protein2
Substrate water, Urea

Cross database links:

Genevestigator: O82598
eggNOG: KOG0223
HEGENOM: HBG705794
RefSeq: NP_192056.1   
Entrez Gene ID: 828051   
Pfam: PF00230   
KEGG: ath:AT4G01470   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005774 C:vacuolar membrane
GO:0015204 F:urea transmembrane transporter activity
GO:0015250 F:water channel activity
GO:0006950 P:response to stress
GO:0055085 P:transmembrane transport
GO:0015840 P:urea transport
GO:0006833 P:water transport

References (4)

[1] “Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.”  Mayer K.F.X.et.al.   10617198
[2] “Simultaneous high-throughput recombinational cloning of open reading frames in closed and open configurations.”  Underwood B.A.et.al.   17147637
[3] “From genome to function: the Arabidopsis aquaporins.”  Quigley F.et.al.   11806824
[4] “Arabidopsis tonoplast proteins TIP1 and TIP2 interact with the cucumber mosaic virus 1a replication protein.”  Kim M.J.et.al.   17030879

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MPINRIAIGT PGEASRPDAI RAAFAEFFSM VIFVFAGQGS GMAYGKLTGD GPATPAGLVA 
61:	ASLSHAFALF VAVSVGANVS GGHVNPAVTF GAFIGGNITL LRAILYWIAQ LLGAVVACLL 
121:	LKVSTGGMET AAFSLSYGVT PWNAVVFEIV MTFGLVYTVY ATAVDPKKGD IGIIAPLAIG 
181:	LIVGANILVG GAFDGASMNP AVSFGPAVVS WIWTNHWVYW VGPFIGAAIA AIVYDTIFIG 
241:	SNGHEPLPSN DF