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1.A.8.10.8
Aquaporin-B, AqpB of 294 aas and 6 TMSs.  Tyr216 in loop D is a key residue in gating, possibly involving phosphorylation. Mutation of Tyr216 to aspartate or glutamate initiated water permeability. The truncated, permanently open AqpB yielded cells with reduced capability to cope with hypotonic stress (von Bülow et al. 2015).

Accession Number:Q54WT8
Protein Name:Aquaporin-B
Length:294
Molecular Weight:31243.00
Species:Dictyostelium discoideum (Slime mold) [44689]
Number of TMSs:6
Location1 / Topology2 / Orientation3: Cell membrane1 / Multi-pass membrane protein2
Substrate water

Cross database links:

eggNOG: KOG0223
Entrez Gene ID: 8622035   
Pfam: PF00230   
KEGG: ddi:DDB_G027944   

Gene Ontology

GO:0016023 C:cytoplasmic membrane-bounded vesicle
GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0005215 F:transporter activity

References (2)

[1] “Functional characterization of a novel aquaporin from Dictyostelium discoideum amoebae implies a unique gating mechanism.”  von Buelow J.et.al.   22262860
[2] “The genome of the social amoeba Dictyostelium discoideum.”  Eichinger L.et.al.   15875012

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MSLKRSDDYQ DLEEGIAMED GGNIKDEEEK PLDPIEEQNK KRWVLIRAVL GELLCTFLFV 
61:	YVLCATSANF IRLGSPPNPV VGGLSTGFAA VALIYSFADV SGAHFNPAVT FATCVTRKTS 
121:	ITKGLMYVGA QLVGSVLASL ILLATFPGNF PGDKNAASAV AIAPSTDANI GNAFLTELVL 
181:	TFILVYVIFA VAFDTVDNSV KTKVVGKSSS NNLTIYTTSG QTKAGFAPIA IGFTLGFLCF 
241:	LGGSVSGGAF NPARVFGTAL VGNNWTRHWM YWIADFLGAG LAGFAQKFFS STHK