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1.A.8.11.4
Plasma membrane intrinsic protein 2a (forms active heterotetramers with PIP1;1 (TC# 1.A.8.11.3); down regulated under drought stress (Najafabadi et al., 2008). Transports H2O2 (Dynowski et al., 2008). The Mesembryanthemum crystallinum PIP2;1 orthologue is an aquaporin impermeable to urea and glycerol. It is positively regulated by PKA- and PKC- mediated phosphorylation (Amezcua-Romero et al., 2010). PIP1;1 and PIP2;2 (Q9ATM8) co-expression modulates the membrane water permeability in the halophyte Beta vulgaris storage root through a pH regulatory response, enhancing membrane versatility to adjust its water transfer capacity (Bellati et al., 2010). The wheat orthologue has been described (Ayadi et al., 2011).  Inter-TMS interactions occurring both within and between monomers play crucial roles in tetramer formation, and assembly of tetramers is critical for their trafficking from the ER to the plasma membrane as well as water permeability (Yoo et al. 2016).  This protein as well as 1.A.8.11.6 is possibly orthologous to spinach PIP1;2 for which the crystal structure is available (PDP# 4JC6) (Berny et al. 2016).  Plays a role in drought and salt tolerance (Wang et al. 2015).

Accession Number:P43286
Protein Name:PIP2;1
Length:287
Molecular Weight:30474.00
Species:(Mouse-ear cress) [3702]
Number of TMSs:6
Location1 / Topology2 / Orientation3: Cell membrane1 / Multi-pass membrane protein2
Substrate H2O2

Cross database links:

Genevestigator: P43286
eggNOG: KOG0223
HEGENOM: HBG705794
RefSeq: NP_001030851.1    NP_190910.1   
Entrez Gene ID: 824510   
Pfam: PF00230   
KEGG: ath:AT3G53420   

Gene Ontology

GO:0009507 C:chloroplast
GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0005773 C:vacuole
GO:0015250 F:water channel activity
GO:0009414 P:response to water deprivation
GO:0055085 P:transmembrane transport
GO:0006833 P:water transport

References (8)

[1] “Water channels in the plant plasma membrane cloned by immunoselection from a mammalian expression system.”  Kammerloher W.et.al.   7920711
[2] “Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.”  Salanoubat M.et.al.   11130713
[3] “Empirical analysis of transcriptional activity in the Arabidopsis genome.”  Yamada K.et.al.   14593172
[4] “Role of a single aquaporin isoform in root water uptake.”  Javot H.et.al.   12566588
[5] “Random GFP::cDNA fusions enable visualization of subcellular structures in cells of Arabidopsis at a high frequency.”  Cutler S.R.et.al.   10737809
[6] “From genome to function: the Arabidopsis aquaporins.”  Quigley F.et.al.   11806824
[7] “Phosphoproteomics of the Arabidopsis plasma membrane and a new phosphorylation site database.”  Nuehse T.S.et.al.   15308754
[8] “Methylation of aquaporins in plant plasma membrane.”  Santoni V.et.al.   16839310

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MAKDVEAVPG EGFQTRDYQD PPPAPFIDGA ELKKWSFYRA VIAEFVATLL FLYITVLTVI 
61:	GYKIQSDTDA GGVDCGGVGI LGIAWAFGGM IFILVYCTAG ISGGHINPAV TFGLFLARKV 
121:	SLPRALLYII AQCLGAICGV GFVKAFQSSY YTRYGGGANS LADGYSTGTG LAAEIIGTFV 
181:	LVYTVFSATD PKRSARDSHV PVLAPLPIGF AVFMVHLATI PITGTGINPA RSFGAAVIYN 
241:	KSKPWDDHWI FWVGPFIGAA IAAFYHQFVL RASGSKSLGS FRSAANV