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1.A.8.9.2
Aquaporin-9 (Aqp9) (permeable to glycerol, urea, polyols, carbamides, purines, pyrmidines, nucleosides, monocarboxylates, pentavalent methylated arsenicals and the arsenic chemotherapeutic drug, trisenox (McDermott et al., 2009).  It is poorly permeable to water and not permeable to β-hydroxybutyrate (Carbrey et al., 2003). (Regulated by CFTR and NHERF1 in response to cyclic AMP (Pietrement et al., 2008)) The 7 Å projection structure and a homology model revealed that pore-lining residues and the hydrophobic edge of the tripathic pore of GlpF (1.A.8.1.1) provide the basis for broad substrate specificity (Viadiu et al., 2007).  Important for urea transport in mouse hepatocytes (Jelen et al. 2012).  Activation of the PPARα transcription factor results in reduction in the abundance of AQP9 in periportal hepatocytes, but its activation in the fed state directs glycerol into glycerolipid synthesis rather than into de novo synthesis of glucose (Lebeck et al. 2015).  Azacytidine up-regulates AQP9 and enhances arsenic trioxide (As2O3)-mediated cytotoxicity in acute myeloid leukemia (AML) (Chau et al. 2015).  Human Aqp9 transports hydrogen peroxide (HOOH) (Watanabe et al. 2016).

Accession Number:P56627
Protein Name:AQP9
Length:295
Molecular Weight:31871.00
Species:Rattus norvegicus (Rat) [10116]
Number of TMSs:6
Location1 / Topology2 / Orientation3: Membrane1 / Multi-pass membrane protein2
Substrate glycerol, Polyols, Carbamides, Purines, Pyrimidines, Monocarboxylates, Nucleosides, Pentavalent methylated arsenicals, Trisenox, water

Cross database links:

Genevestigator: P56627
eggNOG: roNOG04960
RefSeq: NP_075249.1   
Entrez Gene ID: 65054   
Pfam: PF00230   
KEGG: rno:65054   

Gene Ontology

GO:0016323 C:basolateral plasma membrane
GO:0016021 C:integral to membrane
GO:0005792 C:microsome
GO:0015250 F:water channel activity
GO:0015722 P:canalicular bile acid transport
GO:0055085 P:transmembrane transport
GO:0006833 P:water transport

References (3)

[1] “Molecular characterization of a broad selectivity neutral solute channel.”  Tsukaguchi H.et.al.   9733774
[2] “Cloning and functional expression of rAQP9L a new member of aquaporin family from rat liver.”  Ko S.B.et.al.   10205677
[3] “The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).”  The MGC Project Teamet.al.   15489334

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MPSEKDGAKK SLMQRLALKS RIAKETLSEF LGTFIMIVLG CSSIAQAVLS RERFGGIITI 
61:	NIGFASAVVM ALYVTFGISG GHINPAVSFA MCAFGRMEWF KFPFYVGAQF LGAFVGAATV 
121:	FGIYYDGLMA FAGGKLLVVG ENATAFIFAT YPAPFISTPG AFVDQVVSTM FLLLIVFAMF 
181:	DSRNLGVPRG LEPVVIGLLI IVLSCSLGLN SGCAMNPARD LSPRLFTALA GWGFEVFTVG 
241:	NNFWWIPVVG PMIGAFLGGL IYILFIQMHH SKLDPDMKAE PSENNLEKHE LSVIM