TCDB is operated by the Saier Lab Bioinformatics Group
« See all members of the family


1.A.85.1.1
The human poliovirus 2B viroporin (from the polyprotein). The 97 residue 2B viroporin consists of residues 1031-1127 in the polyprotein.  in vitro and in vivo translation-glycosylation compatible with the translocon-mediated insertion of the 2B product into the ER membrane as a double-spanning integral membrane protein with an N-/C-terminal cytoplasmic orientation has been reported (Agirre et al. 2008; Martínez-Gil et al. 2011). The in vitro translation of several truncated versions of the 2B protein suggests that the two hydrophobic regions cooperate to insert into the ER-derived microsomal membranes. The 3A viroporin of poliovirus transports monovalent cations (Hyser and Estes 2015).

Accession Number:Q9Q280
Protein Name:Enterovirus polypeptide
Length:2209
Molecular Weight:246046.00
Species:Human poliovirus 1 [12080]
Location1 / Topology2 / Orientation3: Cell outer membrane1 / Multi-pass membrane protein2
Substrate monovalent cations

Cross database links:

Pfam: PF08727    PF00548    PF02226    PF00947    PF01552    PF00680    PF00073    PF00910   

Gene Ontology

GO:0044161 C:host cell cytoplasmic vesicle
GO:0033644 C:host cell membrane
GO:0016020 C:membrane
GO:0019028 C:viral capsid
GO:0005524 F:ATP binding
GO:0004197 F:cysteine-type endopeptidase activity
GO:0003723 F:RNA binding
GO:0003724 F:RNA helicase activity
GO:0003968 F:RNA-directed RNA polymerase activity
GO:0005198 F:structural molecule activity
GO:0006508 P:proteolysis
GO:0018144 P:RNA-protein covalent cross-linking
GO:0006410 P:transcription, RNA-dependent
GO:0019079 P:viral genome replication

References (2)

[1] “Evolution of poliovirus type I during 5.5 years of prolonged enteral replication in an immunodeficient patient.”  Bellmunt A.et.al.   10600590
[2] “Role of myristoylation of poliovirus capsid protein VP4 as determined by site-directed mutagenesis of its N-terminal sequence.”  Marc D.et.al.   2555183

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
Window Size: Angle:  
Window Size: Angle:  
FASTA formatted sequence
1:	MGAQVSSQKV GAHENSNKAS GGSTINYTTI NYYRDSASNA ASKQDFSQDP SKFTEPIKDV 
61:	LIKTAPTLNS PNIEACGYSD RVMQLTLGNS TITTQEAANS VVAYGRWPEY LRDSEANPVD 
121:	QPTEPDVAAC RFYTLDTVSW TKESRGWWWK LPDALRDMGL FGQNMYYHYL GRSGYTVHVQ 
181:	CNASKFHQGA LGVFAVPEMC LAGDSNTTTM HTSYQNANPG EKGGTFTGTF TPDTNQTSPT 
241:	RRFCPVDYLF GNGTLLGNAF VFPHQIINLR TNNCATLVLP YVNSLSIDSM VKHNNWGIAI 
301:	LPLAPLNFAS ESSTEIPITL TIAPMCCEFN GLRNITLPRL QGLPVMNTPG SNQYLTADNF 
361:	QSPCALPEFD VTPPIDIPGE VKNMMELAEI DTMIPLNLSA TKKNTMDMYR VQLNDNPHTD 
421:	KPILCLSLSP ASDPRLSHTM LGEVLNYYTH WAGSLKFTFL FCGSMMATGK LLVSYAPPGA 
481:	DPPKRRKEAM LGTHVIWDIG LQSSCTMVVP WISNTTYRQT TNDSFTEGGY VSVFYQTRVV 
541:	VPLSTPREMD ILGFVSACND FSVRLLRDTT HIGQSALAQG IGQMLESMID NTVRETAGAA 
601:	TSRNALPNTE ASGSAHSKEI PALTAVETGA TNPLVPSDTV QTRHVIQHRS RSESSIESFF 
661:	ARGACVSIIT VDNSASSAKN DRLFAVWKIT YKDTVQLRRK LEFFTYSRFD MEFTFVVTAN 
721:	FTETNNGHAL NQVYQIMYVP PGAPVPAKWD DYTWQTSSNP SIFYTYGAAP ARISIPYVGI 
781:	ANAYSHFYDG FSKVPLKDQS AALGDSLYGA ASLNDFGVLA VRVVNDHNPT KVTSKIRVYL 
841:	KPKHIRVWCP RPPRAVAYYG PGVDYKDGTL TPLSTKDLTT YGFGHQNKAV YTAGYKICNY 
901:	HLATQEDLQN AVSTMWDRDL LVTESKAQGT DTIARCNCKA GVYYCESRRK YYPVSFIGPT 
961:	FQYMEANGYY PARYQSHILI GHGFASPGDC GGILRCHHGV IGIITAGGEG LVAFADIRDL 
1021:	YAYEEEAMEQ GITNYIESLG AAFGSGFTQQ IGDKVTELTN MVTSTITEKL LKNLIKIISS 
1081:	LVIITRNYED TTTVLATLAL LGCDVSPWQW LKKKACDILE IPYVIKQGDS WLKKFTEACN 
1141:	AAKGLEWVSN KISKFIDWLK EKIIPQARDK LEFVTRLKQL EMLENQISTI HQSCPSQEHQ 
1201:	EVLFNNVRWL SIQSKRFAPL YAMEAKRIQK LEHTINNYIQ FKSKHRIEPV CLLVHGSPGT 
1261:	GKSVATNLIA RAIAERENTS TYSLPPDPSH FDGYKQQGVV IMDDLNQNPD GADMKLFCQM 
1321:	VSTVEFIPPM ASLEEKGILF TSNYVLASTN SSRITPPTVA HSDALTRRFA FDMDIQVMSE 
1381:	YFRDGKLDMT MATEMCKNCH QPANFKRCCP LVCGKAIQLM DKSSRVRYSV DQITTMIVNE 
1441:	RNRRSNIGNC MEALFQGPIQ YKDLKIDIKT SPPPECINDL LQAVDSQEVR NYCEKKGWIV 
1501:	NITSQVQTER NINRAMTILQ AVTTFAAVAG VVYVMYKLFA GHQGAYTGLP NKKPNVPTIR 
1561:	TAKVQGPGFD YAVAMAKRNI VTATTSKGEF TMLGIHDNVA ILPTHASPGE SIVIDGKEVE 
1621:	ILDAKALEDQ AGTNLEITII TLRRNEKFRD IRPHIPTQIT ETNDGVLIVN TSKYPNMFVP 
1681:	VGAVTEQGYL NLGGRQTART LMYNFPTRAG QCGGVITCTG KVIGMHVGGN GSHGFAAALK 
1741:	RSYFTQSQGE IQWMRPSKEV GYPIINAPSK TKLEPSAFHY VFEGVKEPAV LTKSDPRLKT 
1801:	DFEEAIFSKY VGNKITEVDE YMKEAVDHYA GQLMALDINT EQMCLEDAMY GTDGLEALDL 
1861:	STSAGYPYVA MGKKKRDILN KQTRDTKEMQ RLLDTYGINL PLVTYVKDEL RSKAKVEQGK 
1921:	SRLIEASSLN DSVAMRMAFG NLYAAFHKNP GVITGSAVGC DPDLFWSKIP VLMEERLFAF 
1981:	DYTGYDASLS PAWFEALKMV LEKIGFGDRV DYIDYLNHSH HLYKNKTYCV KGGMPSGCSG 
2041:	TSIFNSMINN LIIRTLLLKT YKGIDLDHLK MIAYGDDVIA SYPHEVDASL LAQSGKDYGL 
2101:	TMTPADKSAT FETVTWENVT FLKRFFRADE KYPFLVHPVM PMKEIHESIR WTKDPRNTQD 
2161:	HVRSLCLLAW HNGEEEYNKF LAKIRSVPIG RALLLPEYST LYRRWLDSF