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1.A.9.5.8
Rice stem borer GABA recpetor of 496 aas and 4 TMSs.  Insect GABAR is a major targets of insecticides. cDNAs (CsRDL1A and CsRDL2S) encoding the two isoforms of RDL subunits were cloned from the rice stem borer Chilo suppressalis. Transcripts of both genes demonstrated similar expression patterns in different tissues and developmental stages, although CsRDL2S was approximately 2-fold more abundant than CsRDL1A throughout all development stages. Electrophysiological results using a two-electrode voltage clamp demonstrated that GABA activated currents in oocytes injected with both cRNAs. The EC50 value of GABA in activating currents was smaller in oocytes co-injected with CsRDL1A and CsRDL2S than in oocytes injected singly. The IC50 value of the insecticide fluralaner in inhibiting GABA responses was smaller in oocytes co-injected with different cRNAs than in oocytes injected singly. Co-injection also changed the potency of the insecticide dieldrin in oocytes injected singly. Thus, heteromeric GABARs were formed by the co-injections of CsRDL1A and CsRDL2S in oocytes. Although the presence of Ser at the 2'-position in the second TMS was responsible for the insensitivity of GABARs to dieldrin, this amino acid did not affect the potencies of the insecticides fipronil and fluralaner. Thus, C. suppressalis may adapt to insecticide pressure by regulating the expression levels of CsRDL1A and CsRDL2S and the composition of both subunits in GABARs.

Accession Number:E2IKR3
Protein Name:GABA receptor
Length:496
Molecular Weight:55480.00
Species:Chilo suppressalis (Asiatic rice borer moth) [168631]
Number of TMSs:4
Substrate

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FASTA formatted sequence
1:	MHTSRSRGVH NFALVVALTI AWLSHADHAA GTGGGGMFGD VNISAILDSL SVSYDKRVRP 
61:	NYGGPPVDVG VTMYVLSISS LSEVKMDFTL DFYFRQFWTD PRLAYKKRTG VETLSVGSEF 
121:	IRNIWVPDTF FVNEKQSYFH IATTSNEFIR IHHSGSITRS IRLTITASCP MDLQYFPMDR 
181:	QLCNIEIESF GYTMRDIRYK WNEGPNSVGV SSEVSLPQFK VLGHRQRAME ISLTTGNYSR 
241:	LACEIQFVRS MGYYLIQIYI PSGLIVIISW VSFWLNRNAT PARVSLGVTT VLTMTTLMSS 
301:	TNAALPKISY VKSIDVYLGT CFVMVFASLL EYATVGYMAK RIQMRKQRFT AVQKMAAEKK 
361:	MHIDGPPGTS EPLPPPRTST LNRPLPPSRS SEVRFKVHDP KAYSKGGTLE NTINGARAPP 
421:	PPPVPQPEED PAPPPHLLQA SKGINKLLGT TPSDIDKYSR IVFPVCFVCF NLMYWIIYLH 
481:	VSDVVADDLV LLGEEN