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1.A.94.1.1
Non-structural glycoprotein 4, NSP4 or enterotoxin, of 175 aas and 2 TMSs (Hyser et al. 2012).  A pentatmeric structure of a 53 aas NSP4 fragment has been solved (3MIW).  NSP4 viroporin is involved in activation.  It increases the  endoplasmic reticulum (ER) permeability, resulting in decreased ER calcium stores and activation of plasma membrane (PM) calcium influx channels, ultimately causing the elevation in cytoplasmic calcium (Hyser et al. 2013).  It activates ER calcium store-operated calcium entry (Hyser et al. 2013). NSP4 VPD is a Ca2+/Ba2+-conducting cation-selective viroporin that transports monovalent and divalent cations equally well (Pham et al. 2017).

Accession Number:P04512
Protein Name:Non-structural glycoprotein 4
Length:175
Molecular Weight:20267.00
Species:Rotavirus A (strain SA11-Both G3-P5B[2]-I2-R2-C5-M5-A5-N2-T5-E2-H5) (RV-A) [37137]
Number of TMSs:2
Location1 / Topology2 / Orientation3: Host rough endoplasmic reticulum membrane1 / Single-pass type III membrane protein2
Substrate divalent inorganic cations, calcium ions, monovalent inorganic cations

Cross database links:

Structure:
1G1I   1G1J   2O1K     

External Searches:

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  • 2° Structure (Network Protein Sequence Analysis)

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MEKLTDLNYT LSVITLMNNT LHTILEDPGM AYFPYIASVL TGLFALNKAS IPTMKIALKT 
61:	SKCSYKVVKY CIVTIFNTLL KLAGYKEQIT TKDEIEKQMD RVVKEMRRQL EMIDKLTTRE 
121:	IEQVELLKRI YDKLTVQTTG EIDMTKEINQ KNVRTLEEWE SGKNPYEPRE VTAAM